After two years and more than 1,000 tissue homogenates, we are beginning to quantitate our computer database of profile information in order to further augment our genetic quality assurance program. We can now supplement raw data with sophisticated densitometry of our autoradiograms and gels. Though we are still using biochemical isozymes to routinely check the genetic authenticity of rodents used as experimental models both within and outside NIH, we now regularly supplement these assays with DNA fingerprinting. We have found the technique absolutely essential in testing for genetic purity in both recombinant and congenic strains. It is perhaps the only reliable way to distinguish between certain species of rodents.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Intramural Research (Z01)
Project #
1Z01RR010436-03
Application #
5204117
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1995
Total Cost
Indirect Cost
Name
National Center for Research Resources
Department
Type
DUNS #
City
State
Country
United States
Zip Code