Abstract

A major scientific challenge is to understand the molecular events that drive the evolution of premalignant lesions in actual tissue. Laser capture microdissection (LCM) was originated to provide a reliable method to procure pure populations of cells from specific microscopic regions of tissue sections; in one step, under direct visualization. The cells of interest are transferred to a polymer film that is activated by laser pulses. The exact morphology of the procured cells (with intact DNA, RNA and proteins) is retained and held on the transfer film. LCM technology has been successfully applied to DNA, and RNA analysis from frozen and fixed embedded tissue. In the past it has not been possible to extract, quantify and characterize the functional state of specific proteins expressed by individual subpopulations of cells in actual tissue. Consequently, an important ongoing and future goal is to extend our microdissection technology to include the molecular profiling of cancer progression into the realm of quantitative proteomics: characterization of known proteins, as well as discovery of new proteins associated with progression.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Division of Clinical Sciences - NCI (NCI)
Type
Intramural Research (Z01)
Project #
1Z01SC009185-14
Application #
6947612
Study Section
(LP)
Project Start
Project End
Budget Start
Budget End
Support Year
14
Fiscal Year
2003
Total Cost
Indirect Cost

  Institution

Name
Clinical Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Zhou, Ming; Lucas, David A; Chan, King C et al. (2004) An investigation into the human serum ""interactome"". Electrophoresis 25:1289-98
Ornstein, David K; Rayford, Walter; Fusaro, Vincent A et al. (2004) Serum proteomic profiling can discriminate prostate cancer from benign prostates in men with total prostate specific antigen levels between 2.5 and 15.0 ng/ml. J Urol 172:1302-5
Interewicz, B; Olszewski, W L; Leak, L V et al. (2004) Profiling of normal human leg lymph proteins using the 2-D electrophoresis and SELDI-TOF mass spectrophotometry approach. Lymphology 37:65-72
Petricoin, Emanuel F; Ornstein, David K; Liotta, Lance A (2004) Clinical proteomics: Applications for prostate cancer biomarker discovery and detection. Urol Oncol 22:322-8
Liotta, Lance A; Kohn, Elise (2004) Anoikis: cancer and the homeless cell. Nature 430:973-4
Krieg, Rene C; Knuechel, Ruth; Schiffmann, Elliot et al. (2004) Mitochondrial proteome: cancer-altered metabolism associated with cytochrome c oxidase subunit level variation. Proteomics 4:2789-95
Johann Jr, Donald J; McGuigan, Michael D; Patel, Amit R et al. (2004) Clinical proteomics and biomarker discovery. Ann N Y Acad Sci 1022:295-305
Espina, Virginia; Woodhouse, Elisa C; Wulfkuhle, Julia et al. (2004) Protein microarray detection strategies: focus on direct detection technologies. J Immunol Methods 290:121-33
Geho, David H; Lahar, Nicholas; Ferrari, Mauro et al. (2004) Opportunities for nanotechnology-based innovation in tissue proteomics. Biomed Microdevices 6:231-9
Petricoin, Emanuel F; Fishman, David A; Conrads, Thomas P et al. (2004) Lessons from Kitty Hawk: from feasibility to routine clinical use for the field of proteomic pattern diagnostics. Proteomics 4:2357-60

Showing the most recent 10 out of 31 publications