Abstract

We are studying a series of patients with a preliminary diagnosis of large granular lymphoproliferative disorder (LGLPD) using flow cytometric immunophenotyping as well as PCR and restriction enzyme digests for clonal T-cell populations. We have found that presence of cytopenias and observation of large granular lymphocytes in the peripheral blood are not sensitive indicators of LGLPD. We have detected B-cell neoplasms and non-cytotoxic T-cell proliferations (non-clonal) and malignancies in patients with cytopenias and peripheral blood large granular lymphocytes. Flow cytometric analysis is more sensitive than PCR (reported 60-70% sensitivity) in detecting T-cell malignancies in LGLPD. Restriction enzyme digests are being performed and sensitivity of detection will be compared. We are using multiparametric approaches to improve the sensitivity of detection of monoclonal B-cell populations. By targeting abnormal patterns of antigen expression (eg CD10, CD5, CD23, FMC7) by B-cells in light chain detection, we are attempting to achieve a pure tumor gate among admixed polyclonal B-cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Intramural Research (Z01)
Project #
1Z01SC009372-05
Application #
2464521
Study Section
Special Emphasis Panel (LP)
Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
1996
Total Cost
Indirect Cost

  Institution

Name
National Cancer Institute Division of Clinical Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

O'Mahony, Deirdre; Morris, John C; Stetler-Stevenson, Maryalice et al. (2009) EBV-related lymphoproliferative disease complicating therapy with the anti-CD2 monoclonal antibody, siplizumab, in patients with T-cell malignancies. Clin Cancer Res 15:2514-22
Stetler-Stevenson, M; Yuan, C M (2009) Myelodysplastic syndromes: the role of flow cytometry in diagnosis and prognosis. Int J Lab Hematol 31:479-83
Arons, Evgeny; Sorbara, Lynn; Raffeld, Mark et al. (2006) Characterization of T-cell repertoire in hairy cell leukemia patients before and after recombinant immunotoxin BL22 therapy. Cancer Immunol Immunother 55:1100-10
Schinstine, Malcolm; Filie, Armando C; Wilson, Wyndham et al. (2006) Detection of malignant hematopoietic cells in cerebral spinal fluid previously diagnosed as atypical or suspicious. Cancer 108:157-62
Arons, Evgeny; Margulies, Inger; Sorbara, Lynn et al. (2006) Minimal residual disease in hairy cell leukemia patients assessed by clone-specific polymerase chain reaction. Clin Cancer Res 12:2804-11
Fogarty, Patrick F; Stetler-Stevenson, Maryalice; Pereira, Aloysius et al. (2005) Large granular lymphocytic proliferation-associated cyclic thrombocytopenia. Am J Hematol 79:334-6
Lamb Jr, Lawrence S; Neuberg, Ronnie; Welsh, Jeff et al. (2005) T-cell lymphoblastic leukemia/lymphoma syndrome with eosinophilia and acute myeloid leukemia. Cytometry B Clin Cytom 65:37-41
Ahmad, Ejaz; Kingma, Douglas W; Jaffe, Elaine S et al. (2005) Flow cytometric immunophenotypic profiles of mature gamma delta T-cell malignancies involving peripheral blood and bone marrow. Cytometry B Clin Cytom 67:6-12
Hegde, Upendra; Filie, Armando; Little, Richard F et al. (2005) High incidence of occult leptomeningeal disease detected by flow cytometry in newly diagnosed aggressive B-cell lymphomas at risk for central nervous system involvement: the role of flow cytometry versus cytology. Blood 105:496-502
Marti, Gerald E; Vogt Jr, Robert F; Stetler-Stevenson, Maryalice (2003) Clinical quantitative flow cytometry: ""Identifying the optimal methods for clinical quantitative flow cytometry"". Cytometry B Clin Cytom 55:59

Showing the most recent 10 out of 13 publications