""""""""Fluorescent pteridine-based nucleoside analogs that can be site-specifically incorporated into DNA oligonucleotides through a phosphodiester linkage have been developed and patented. The fluorescence properties of a series of these compounds have been characterized and potential applications are being investigated. These new fluorophores are promising probes for the study of protein/DNA interactions, because they are not attached to DNA externally and they do not appear to disrupt the tertiary structure of DNA. Rapid fluorescence-based assays for HIV-1 integrase and alkylguanine-DNA-akyltransferase have been developed, and the potential utility of these fluorophores as hybridization probes has been patented and is under investigation.""""""""

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Intramural Research (Z01)
Project #
1Z01SC010090-02
Application #
6123755
Study Section
Special Emphasis Panel (POB)
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1998
Total Cost
Indirect Cost
Name
National Cancer Institute Division of Clinical Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Hawkins, Mary E (2007) Synthesis, purification and sample experiment for fluorescent pteridine-containing DNA: tools for studying DNA interactive systems. Nat Protoc 2:1013-21
Turingan, Rosemary S; Liu, Cuihua; Hawkins, Mary E et al. (2007) Structural confirmation of a bent and open model for the initiation complex of T7 RNA polymerase. Biochemistry 46:1714-23
Hawkins, Mary E; Balis, Frank M (2004) Use of pteridine nucleoside analogs as hybridization probes. Nucleic Acids Res 32:e62