Abstract

The first step in virus replication is entry. The mechanisms used by poxviruses are complicated by the existence of two distinct infectious forms with different external membranes. Another complicating factor is the ability of vaccinia virus to enter cells through the plasma membrane and by endocytosis. We have identified a complex comprised of at least 12 proteins that are required for entry of both virus forms. These proteins are conserved in all members of the poxvirus family, suggesting a common entry mechanism.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAI000539-25
Application #
8555773
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
25
Fiscal Year
2012
Total Cost
$608,545
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Moss, Bernard (2016) Membrane fusion during poxvirus entry. Semin Cell Dev Biol 60:89-96
Gjessing, Mona C; Yutin, Natalya; Tengs, Torstein et al. (2015) Salmon Gill Poxvirus, the Deepest Representative of the Chordopoxvirinae. J Virol 89:9348-67
Moss, Bernard (2015) Poxvirus membrane biogenesis. Virology 479-480:619-26
Laliberte, Jason P; Moss, Bernard (2014) A novel mode of poxvirus superinfection exclusion that prevents fusion of the lipid bilayers of viral and cellular membranes. J Virol 88:9751-68
Satheshkumar, P S; Chavre, James; Moss, Bernard (2013) Role of the vaccinia virus O3 protein in cell entry can be fulfilled by its Sequence flexible transmembrane domain. Virology 444:148-57
Helenius, Ari; Moss, Bernard (2013) Virus entry--an unwilling collaboration by the cell. Curr Opin Virol 3:1-2
Wolfe, Cindy L; Ojeda, Suany; Moss, Bernard (2012) Transcriptional repression and RNA silencing act synergistically to demonstrate the function of the eleventh component of the vaccinia virus entry-fusion complex. J Virol 86:293-301
Satheshkumar, P S; Moss, Bernard (2012) Sequence-divergent chordopoxvirus homologs of the o3 protein maintain functional interactions with components of the vaccinia virus entry-fusion complex. J Virol 86:1696-705
Bengali, Zain; Satheshkumar, P S; Moss, Bernard (2012) Orthopoxvirus species and strain differences in cell entry. Virology 433:506-12
Moss, Bernard (2012) Poxvirus cell entry: how many proteins does it take? Viruses 4:688-707

Showing the most recent 10 out of 19 publications