Abstract

LID scientists are collaborating with scientists from MedImmune under a CRADA to generate candidate vaccines against pandemic influenza viruses, including the 2009 pandemic H1N1 virus. The vaccine was generated using plasmid based reverse genetics and contains the hemagglutinin and neuraminidase genes from the pandemic H1N1 influenza virus and six internal gene segments from the AA ca virus. The immunogenicity and efficacy of the vaccine will be evaluated in animal models. The serum antibody response to one or two doses of vaccine will be evaluated using hemagglutination inhibition and neutralizing antibody assays and the efficacy will be evaluated by challenging vaccinated animals with the wild-type H1N1 pandemic influenza virus.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAI001109-01
Application #
7964771
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2009
Total Cost
$45,876
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Czakó, Rita; Vogel, Leatrice; Lamirande, Elaine W et al. (2017) In Vivo Imaging of Influenza Virus Infection in Immunized Mice. MBio 8:
Broadbent, Andrew J; Santos, Celia P; Anafu, Amanda et al. (2016) Evaluation of the attenuation, immunogenicity, and efficacy of a live virus vaccine generated by codon-pair bias de-optimization of the 2009 pandemic H1N1 influenza virus, in ferrets. Vaccine 34:563-570
Lakdawala, Seema S; Jayaraman, Akila; Halpin, Rebecca A et al. (2015) The soft palate is an important site of adaptation for transmissible influenza viruses. Nature 526:122-5
O'Donnell, Christopher D; Wright, Amber; Vogel, Leatrice et al. (2014) Humans and ferrets with prior H1N1 influenza virus infections do not exhibit evidence of original antigenic sin after infection or vaccination with the 2009 pandemic H1N1 influenza virus. Clin Vaccine Immunol 21:737-46
Broadbent, Andrew J; Santos, Celia P; Godbout, Rachel A et al. (2014) The temperature-sensitive and attenuation phenotypes conferred by mutations in the influenza virus PB2, PB1, and NP genes are influenced by the species of origin of the PB2 gene in reassortant viruses derived from influenza A/California/07/2009 and A/WSN/ J Virol 88:12339-47
Lakdawala, Seema S; Wu, Yicong; Wawrzusin, Peter et al. (2014) Influenza a virus assembly intermediates fuse in the cytoplasm. PLoS Pathog 10:e1003971
Lakdawala, Seema S; Shih, Angela R; Jayaraman, Akila et al. (2013) Receptor specificity does not affect replication or virulence of the 2009 pandemic H1N1 influenza virus in mice and ferrets. Virology 446:349-56
Boonnak, Kobporn; Vogel, Leatrice; Orandle, Marlene et al. (2013) Antigen-activated dendritic cells ameliorate influenza A infections. J Clin Invest 123:2850-61
Harris, Audray K; Meyerson, Joel R; Matsuoka, Yumiko et al. (2013) Structure and accessibility of HA trimers on intact 2009 H1N1 pandemic influenza virus to stem region-specific neutralizing antibodies. Proc Natl Acad Sci U S A 110:4592-7
O'Donnell, Christopher D; Wright, Amber; Vogel, Leatrice N et al. (2012) Effect of priming with H1N1 influenza viruses of variable antigenic distances on challenge with 2009 pandemic H1N1 virus. J Virol 86:8625-33

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