Abstract

We have two projects. 1. How does CXCR4 receptor signaling to Elmo/Dock complex leading to actin cytoskeleton? Our study in D. discoideum revealed a novel pathway linking G-protein subunits directly to Elmo/Dock complex. We are in the process to investigate whether this is evolutionarily conserved in chemokine receptor-controlled cell migration in human. 2. The role of beta-arrestin in neutrophil chemotaxis. It is known that activated chemokine GPCRs interact with beta-arrestin, which brings signaling components and activates pathways independent of G-proteins. It has been suggested that beta-arrestin signaling plays a role in cell migration, but the molecular mechanisms underlining this process are not known. We are in the process to study these mechanisms using neutrophils.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAI001156-01
Application #
8336363
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2011
Total Cost
$384,770
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Gera, Nidhi; Swanson, Kenneth D; Jin, Tian (2017) ?-Arrestin 1-dependent regulation of Rap2 is required for fMLP-stimulated chemotaxis in neutrophil-like HL-60 cells. J Leukoc Biol 101:239-251
Wang, Gang; Cao, Luyang; Liu, Xiaowen et al. (2016) Oxidant Sensing by TRPM2 Inhibits Neutrophil Migration and Mitigates Inflammation. Dev Cell 38:453-62
Xu, Xuehua; Yun, Michelle; Wen, Xi et al. (2016) Quantitative Monitoring Spatiotemporal Activation of Ras and PKD1 Using Confocal Fluorescent Microscopy. Methods Mol Biol 1407:307-23
Wang, Youhong; Xu, Xuehua; Pan, Miao et al. (2016) ELMO1 Directly Interacts with G?? Subunit to Transduce GPCR Signaling to Rac1 Activation in Chemotaxis. J Cancer 7:973-83
Xu, Xuehua; Gera, Nidhi; Li, Hongyan et al. (2015) GPCR-mediated PLC??/PKC?/PKD signaling pathway regulates the cofilin phosphatase slingshot 2 in neutrophil chemotaxis. Mol Biol Cell 26:874-86
Guo, Jia; Xu, Xuehua; Rasheed, Taban K et al. (2013) Genistein interferes with SDF-1- and HIV-mediated actin dynamics and inhibits HIV infection of resting CD4 T cells. Retrovirology 10:62
Li, Hongyan; Yang, Lei; Fu, Hui et al. (2013) Association between Gýýi2 and ELMO1/Dock180 connects chemokine signalling with Rac activation and metastasis. Nat Commun 4:1706
Guo, Jia; Xu, Xuehua; Yuan, Wen et al. (2012) HIV gp120 is an aberrant chemoattractant for blood resting CD4 T cells. Curr HIV Res 10:636-42
Chen, Yan; Yue, Shen; Xie, Lu et al. (2011) Dual Phosphorylation of suppressor of fused (Sufu) by PKA and GSK3beta regulates its stability and localization in the primary cilium. J Biol Chem 286:13502-11
Kim, Jung-Sik; Xu, Xuehua; Li, Huifang et al. (2011) Mechanistic analysis of a DNA damage-induced, PTEN-dependent size checkpoint in human cells. Mol Cell Biol 31:2756-71