Abstract

Individual gp160 clones were screened for infectivity as Env-pseudotyped viruses in a luciferase reporter gene assay in JC53-BL (TZM-bl) cells. Functional env clones were sequenced and their neutralization phenotypes characterized by using soluble CD4, monoclonal antibodies and serum samples from infected individuals and non-infected recipients of a recombinant gp120 vaccine. Env clones from geographically diverse HIV-1 isolates were selected that were not unusually sensitive or resistant to neutralization and comprised a wide spectrum of genetic, antigenic and geographic diversity 3. These reference reagents will facilitate proficiency testing and other validation efforts aimed at improving assay performance across laboratories and can be used for standardized assessments of vaccine elicited neutralizing antibodies. Using the reference virus reagents described above, the Humoral immunology Core Section continues to screen hundreds of animal sera and antibodies generated by investigators at the VRC as well as extramural collaborators.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAI005010-17
Application #
9784476
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
17
Fiscal Year
2018
Total Cost
Indirect Cost

  Institution

Name
Niaid Extramural Activities
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Bonsignori, Mattia; Kreider, Edward F; Fera, Daniela et al. (2017) Staged induction of HIV-1 glycan-dependent broadly neutralizing antibodies. Sci Transl Med 9:
Williams, LaTonya D; Ofek, Gilad; Schätzle, Sebastian et al. (2017) Potent and broad HIV-neutralizing antibodies in memory B cells and plasma. Sci Immunol 2:
Julg, Boris; Sok, Devin; Schmidt, Stephen D et al. (2017) Protective Efficacy of Broadly Neutralizing Antibodies with Incomplete Neutralization Activity against Simian-Human Immunodeficiency Virus in Rhesus Monkeys. J Virol 91:
Doria-Rose, Nicole A; Altae-Tran, Han R; Roark, Ryan S et al. (2017) Mapping Polyclonal HIV-1 Antibody Responses via Next-Generation Neutralization Fingerprinting. PLoS Pathog 13:e1006148
Hraber, Peter; Rademeyer, Cecilia; Williamson, Carolyn et al. (2017) Panels of HIV-1 Subtype C Env Reference Strains for Standardized Neutralization Assessments. J Virol 91:
Wagh, Kshitij; Bhattacharya, Tanmoy; Williamson, Carolyn et al. (2016) Optimal Combinations of Broadly Neutralizing Antibodies for Prevention and Treatment of HIV-1 Clade C Infection. PLoS Pathog 12:e1005520
Doria-Rose, Nicole A; Schramm, Chaim A; Gorman, Jason et al. (2014) Developmental pathway for potent V1V2-directed HIV-neutralizing antibodies. Nature 509:55-62
Hraber, Peter; Seaman, Michael S; Bailer, Robert T et al. (2014) Prevalence of broadly neutralizing antibody responses during chronic HIV-1 infection. AIDS 28:163-9
deCamp, Allan; Hraber, Peter; Bailer, Robert T et al. (2014) Global panel of HIV-1 Env reference strains for standardized assessments of vaccine-elicited neutralizing antibodies. J Virol 88:2489-507
Georgiev, Ivelin S; Doria-Rose, Nicole A; Zhou, Tongqing et al. (2013) Delineating antibody recognition in polyclonal sera from patterns of HIV-1 isolate neutralization. Science 340:751-6

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