Abstract

As an effort to obtain specimens that might be useful in the study of the presence on specific antigens in tumor types, anonymous tissue donations were accepted. We described a method to generate multitumor blocks without specific equipment. With these tissues, we characterized the distribution of several endothelial antigens: Claudin 5, PROX1, and VEGFR2 (KDR gene product). Also distribution of KBA62 and PNL 2 antigens in melanoma and other tumors was examined. We found VEGFR2 highly specific for endothelial tumors among mesenchymal cancers, but malignant mesotheliomas were found to be unique epithelial cancers strongly positive for VEGFR2. We also examined succinate dehydrogenase deficient gastrointestinal stromal tumors, in collaboration with NCI's pediatric GIST clinic.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIABC011427-02
Application #
8763517
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2013
Total Cost
$270,710
Indirect Cost

  Institution

Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Miettinen, Markku; Felisiak-Golabek, Anna; Wang, Zengfeng et al. (2017) GIST Manifesting as a Retroperitoneal Tumor: Clinicopathologic Immunohistochemical, and Molecular Genetic Study of 112 Cases. Am J Surg Pathol 41:577-585
Inaguma, Shingo; Lasota, Jerzy; Wang, Zengfeng et al. (2017) Clinicopathologic profile, immunophenotype, and genotype of CD274 (PD-L1)-positive colorectal carcinomas. Mod Pathol 30:278-285
Inaguma, Shingo; Wang, Zengfeng; Lasota, Jerzy et al. (2017) Comprehensive immunohistochemical study of mesothelin (MSLN) using different monoclonal antibodies 5B2 and MN-1 in 1562 tumors with evaluation of its prognostic value in malignant pleural mesothelioma. Oncotarget 8:26744-26754
Lasota, Jerzy; Kowalik, Artur; Felisiak-Golabek, Anna et al. (2017) SP174, NRAS Q61R Mutant-Specific Antibody, Cross-Reacts With KRAS Q61R Mutant Protein in Colorectal Carcinoma. Arch Pathol Lab Med 141:564-568
Inaguma, Shingo; Wang, Zengfeng; Lasota, Jerzy P et al. (2016) Expression of neural cell adhesion molecule L1 (CD171) in neuroectodermal and other tumors. An immunohistochemical study of 5155 tumors and critical evaluation of CD171 prognostic value in gastrointestinal stromal tumors. Oncotarget :
Lasota, Jerzy; Felisiak-Golabek, Anna; Wasag, Bartosz et al. (2016) Frequency and clinicopathologic profile of PIK3CA mutant GISTs: molecular genetic study of 529 cases. Mod Pathol 29:275-82
Miettinen, Markku; Felisiak-Golabek, Anna; Wasag, Bartosz et al. (2016) Fumarase-deficient Uterine Leiomyomas: An Immunohistochemical, Molecular Genetic, and Clinicopathologic Study of 86 Cases. Am J Surg Pathol 40:1661-1669
Inaguma, Shingo; Wang, Zengfeng; Lasota, Jerzy et al. (2016) Comprehensive Immunohistochemical Study of Programmed Cell Death Ligand 1 (PD-L1): Analysis in 5536 Cases Revealed Consistent Expression in Trophoblastic Tumors. Am J Surg Pathol 40:1133-42
Kerkar, Sid P; Wang, Zeng-Feng; Lasota, Jerzy et al. (2016) MAGE-A is More Highly Expressed Than NY-ESO-1 in a Systematic Immunohistochemical Analysis of 3668 Cases. J Immunother 39:181-7
Kopczynski, Janusz; Kowalik, Artur; Ch?opek, Ma?gorzata et al. (2016) Oncogenic Activation of the Wnt/?-Catenin Signaling Pathway in Signet Ring Stromal Cell Tumor of the Ovary. Appl Immunohistochem Mol Morphol 24:e28-33

Showing the most recent 10 out of 33 publications