Abstract

We will continue to evaluate the role of steroid profile and thyroid hormone profile monitoring in optimizing both diagnosis and treatment of different endocrine diseases. We developed a state of the art micro assay for 11 steroids by LCMSMS. This assay has been used to optimize the treatment of patients with CAH (Dr Merke, collaborator). We have also shown that there is a statistically significant diurnal fluctuation in steroid concentrations for all steroids tested except progesterone. The extent of this diurnal variation is so large that it clearly necessitates development of new time dependent reference intervals. Manuscript published in Clinical Chemistry and J Steroid Biochem Mol Biol. We will also examine the role of our new free steroid profile and free 25 OH Vitamin D3 assays in health and disease. We have also measured thyroid hormone concentrations, choline and neurosteroid concentrations throughout pregnancy. This work has been submitted for publication. The work described above has been published in numerous papers, a few of which are listed below.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Clinical Center (CLC)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIACL010355-04
Application #
9363641
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2016
Total Cost
Indirect Cost

  Institution

Name
Clinical Center
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Sun, Qian; Gu, Jianghong; Stolze, Brian R et al. (2018) Atmospheric Pressure Chemical Ionization Is a Suboptimal Ionization Source for Steroids. Clin Chem 64:974-976
Ling, C; Sun, Q; Khang, J et al. (2018) Does TSH Reliably Detect Hypothyroid Patients? Ann Thyroid Res 4:122-125
Mallappa, Ashwini; Nella, Aikaterini A; Sinaii, Ninet et al. (2018) Long-term use of continuous subcutaneous hydrocortisone infusion therapy in patients with congenital adrenal hyperplasia. Clin Endocrinol (Oxf) 89:399-407
Hannah-Shmouni, Fady; Soldin, Steven J (2017) Thyroid Hormone Therapy for Older Adults with Subclinical Hypothyroidism. N Engl J Med 377:e20
Welsh, Kerry J; Stolze, Brian R; Yu, Xiaolin et al. (2017) Assessment of thyroid function in intensive care unit patients by liquid chromatography tandem mass spectrometry methods. Clin Biochem 50:318-322
Nilubol, Naris; Soldin, Steven J; Patel, Dhaval et al. (2017) 11-Deoxycortisol may be superior to cortisol in confirming a successful adrenal vein catheterization without cosyntropin: a pilot study. Int J Endocr Oncol 4:75-83
Stolze, Brian R; Gounden, Verena; Gu, Jianghong et al. (2016) An improved micro-method for the measurement of steroid profiles by APPI-LC-MS/MS and its use in assessing diurnal effects on steroid concentrations and optimizing the diagnosis and treatment of adrenal insufficiency and CAH. J Steroid Biochem Mol Biol 162:110-6
Nella, Aikaterini A; Mallappa, Ashwini; Perritt, Ashley F et al. (2016) A Phase 2 Study of Continuous Subcutaneous Hydrocortisone Infusion in Adults with Congenital Adrenal Hyperplasia. J Clin Endocrinol Metab :jc20161916
Welsh, Kerry J; Soldin, Steven J (2016) DIAGNOSIS OF ENDOCRINE DISEASE: How reliable are free thyroid and total T3 hormone assays? Eur J Endocrinol 175:R255-R263
Masika, Likhona Siphe; Zhao, Zhen; Soldin, Steven John (2016) Is measurement of TT3 by immunoassay reliable at low concentrations? A comparison of the Roche Cobas 6000 vs. LC-MSMS. Clin Biochem 49:846-9

Showing the most recent 10 out of 28 publications