Abstract

The recently formed Glia-Neuron Interaction Lab is examining how rodent microglia respond to methamphetamine in both a chronic and acute exposure model. Ongoing work has identified dosing of methamphetamine that cuases unique activation of microglia in the striatum that can be used to examine how activated microglia can affect the surround neurons. A second ongoing project that has been conducted in collaboration with Dr. Jonathan Karn at Case Western University has show that methamphetamine can act directly on a human microglial cell line to activate NFkappaB signaling and increase the transcriptional activity of the HIV LTR promoter. Ongoing studies will examine the signaling by which methamphetamine can influence the NFkB signaling and HIV transcription in microglia.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIADA000586-02
Application #
8736783
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2013
Total Cost
$460,579
Indirect Cost

  Institution

Name
National Institute on Drug Abuse
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Dedoni, Simona; Campbell, Lee A; Harvey, Brandon K et al. (2018) The orphan G-protein-coupled receptor 75 signaling is activated by the chemokine CCL5. J Neurochem 146:526-539
Wallace, Veronica J; Cimbro, Raffaello; Rubio, F Javier et al. (2017) Neurons Internalize Functionalized Micron-Sized Silicon Dioxide Microspheres. Cell Mol Neurobiol 37:1487-1499
Howard, Douglas B; Harvey, Brandon K (2017) Assaying the Stability and Inactivation of AAV Serotype 1 Vectors. Hum Gene Ther Methods 28:39-48
Anttila, Jenni E; Whitaker, Keith W; Wires, Emily S et al. (2017) Role of microglia in ischemic focal stroke and recovery: focus on Toll-like receptors. Prog Neuropsychopharmacol Biol Psychiatry 79:3-14
Campbell, Lee A; Richie, Christopher T; Zhang, Yajun et al. (2017) In vitro modeling of HIV proviral activity in microglia. FEBS J 284:4096-4114
Kuo, Chi-Chung; Shen, Hui; Harvey, Brandon K et al. (2016) Differential modulation of methamphetamine-mediated behavioral sensitization by overexpression of Mu opioid receptors in nucleus accumbens and ventral tegmental area. Psychopharmacology (Berl) 233:661-72
Calu, Donna J; Kawa, Alex B; Marchant, Nathan J et al. (2013) Optogenetic inhibition of dorsal medial prefrontal cortex attenuates stress-induced reinstatement of palatable food seeking in female rats. J Neurosci 33:214-26
Wires, Emily S; Alvarez, David; Dobrowolski, Curtis et al. (2012) Methamphetamine activates nuclear factor kappa-light-chain-enhancer of activated B cells (NF-?B) and induces human immunodeficiency virus (HIV) transcription in human microglial cells. J Neurovirol 18:400-10