Abstract

Using our custom developed miniScope system, we imaged neuronal activity in dorsal striatum in freely moving mice, we also recorded mice locomotor activity, and compared neuronal activity to mice locomotor activity. We found that direct and indirect pathway neurons in the dorsal striatum displayed similar population activity. We further construct neuron cluster models based on our calcium recordings, and uncovered functional heterogeneity amount direct and indirect pathway neurons in dorsal striatum. Going forward, we will use this imaging technique to study how cocaine, THC and other substances of abuse alters dorsal striatum neuronal circuit function, and their contribution in the development of long-term drug addiction and relapse

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIADA000603-04
Application #
9770327
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2018
Total Cost
Indirect Cost

  Institution

Name
National Institute on Drug Abuse
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Chun, Lani S; Vekariya, Rakesh H; Free, R Benjamin et al. (2018) Structure-Activity Investigation of a G Protein-Biased Agonist Reveals Molecular Determinants for Biased Signaling of the D2 Dopamine Receptor. Front Synaptic Neurosci 10:2
Abulseoud, Osama A; Zuccoli, Maria Laura; Zhang, Lifeng et al. (2017) The acute effect of cannabis on plasma, liver and brain ammonia dynamics, a translational study. Eur Neuropsychopharmacol 27:679-690
Etzler, Julie C; Bollo, Mariana; Holstein, Deborah et al. (2017) Cyclophilin D over-expression increases mitochondrial complex III activity and accelerates supercomplex formation. Arch Biochem Biophys 613:61-68
Zhang, Wen; Daly, Kathryn M; Liang, Bo et al. (2016) BDNF rescues prefrontal dysfunction elicited by pyramidal neuron-specific DTNBP1 deletion in vivo. J Mol Cell Biol :
Zhang, Wen; Zhang, Lifeng; Liang, Bo et al. (2016) Hyperactive somatostatin interneurons contribute to excitotoxicity in neurodegenerative disorders. Nat Neurosci 19:557-559
Barbera, Giovanni; Liang, Bo; Zhang, Lifeng et al. (2016) Spatially Compact Neural Clusters in the Dorsal Striatum Encode Locomotion Relevant Information. Neuron 92:202-213
Gu, Yi; Chiu, Shu-Ling; Liu, Bian et al. (2016) Differential vesicular sorting of AMPA and GABAA receptors. Proc Natl Acad Sci U S A 113:E922-31
Lee, Steven T; Williams, Pete A; Braine, Catherine E et al. (2015) A Miniature, Fiber-Coupled, Wireless, Deep-Brain Optogenetic Stimulator. IEEE Trans Neural Syst Rehabil Eng 23:655-64
Daly, Kathryn M; Li, Yun; Lin, Da-Ting (2015) Imaging the Insertion of Superecliptic pHluorin-Labeled Dopamine D2 Receptor Using Total Internal Reflection Fluorescence Microscopy. Curr Protoc Neurosci 70:5.31.1-20
Dickson, Price E; Ndukum, Juliet; Wilcox, Troy et al. (2015) Association of novelty-related behaviors and intravenous cocaine self-administration in Diversity Outbred mice. Psychopharmacology (Berl) 232:1011-24

Showing the most recent 10 out of 12 publications