Investigation of Humans with Informative Iron or Erythroid Phenotypes.

Miller, Jeffery

Abstract

Clinical studies with new technologies are needed to bridge basic and clinical knowledge of erythroid biology and related iron biology consistent with Roadmap goal of the NIH. These studies should enhance clinical activities within the intramural program aimed toward erythroid diseases. The current focus includes recruitment and study of patients with hemoglobinopathies, persistent fetal hemoglobin production, iron deficiency and iron overload. In addition, patient volunteers with other rare phenotypes are recruited for study. In addition to clinical assessment, blood samples are collected for laboratory and research purposes. One major focus of this project is the integration of clinical phenotype with genetic and genomic analyses aimed toward novel diagnostic, prognostic, and therapeutic approaches. In addition to patients seen on the intramural campus, national and international collaborative projects are being pursued in this regard.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
• Type: Investigator-Initiated Intramural Research Projects (ZIA)
• Project #: 1ZIADK025094-05
• Application #: 8349682
• Support Year: 5
• Fiscal Year: 2011
• Total Cost: $159,040

Institution

• Name: National Institute of Diabetes and Digestive and Kidney Diseases

Publications

Rabel, Antoinette; Leitman, Susan F; Miller, Jeffery L (2016) Ask about ice, then consider iron. J Am Assoc Nurse Pract 28:116-20

Byrnes, Colleen; Lee, Y Terry; Meier, Emily R et al. (2016) Iron dose-dependent differentiation and enucleation of human erythroblasts in serum-free medium. J Tissue Eng Regen Med 10:E84-9

Glushakova, Svetlana; Balaban, Amanda; McQueen, Philip G et al. (2014) Hemoglobinopathic erythrocytes affect the intraerythrocytic multiplication of Plasmodium falciparum in vitro. J Infect Dis 210:1100-9

Meier, Emily Riehm; Wright, Elizabeth C; Miller, Jeffery L (2014) Reticulocytosis and anemia are associated with an increased risk of death and stroke in the newborn cohort of the Cooperative Study of Sickle Cell Disease. Am J Hematol 89:904-6

Lee, Y Terry; de Vasconcellos, Jaira F; Yuan, Joan et al. (2013) LIN28B-mediated expression of fetal hemoglobin and production of fetal-like erythrocytes from adult human erythroblasts ex vivo. Blood 122:1034-41

Lee, Y Terry; Kim, Ki Soon; Byrnes, Colleen et al. (2013) A synthetic model of human beta-thalassemia erythropoiesis using CD34+ cells from healthy adult donors. PLoS One 8:e68307

Miller, Jeffery L (2013) Iron deficiency anemia: a common and curable disease. Cold Spring Harb Perspect Med 3:

Meier, Emily Riehm; Byrnes, Colleen; Lee, Y Terry et al. (2013) Increased Reticulocytosis during Infancy Is Associated with Increased Hospitalizations in Sickle Cell Anemia Patients during the First Three Years of Life. PLoS One 8:e70794

Meier, Emily Riehm; Miller, Jeffery L (2012) Sickle cell disease in children. Drugs 72:895-906

Abraham, Bindu; Hicks, Wayne; Jia, Yiping et al. (2011) Isolated Hb Providence ýý82Asn and ýý82Asp fractions are more stable than native HbA(0) under oxidative stress conditions. Biochemistry 50:9752-66