Abstract

Progress in FY2017 was in the following areas: SEGMENTAL LABELING OF HIV-1 CAPSID ASSEMBLIES: We have developed an intein-based protocol for producing the HIV-1 capsid protein (CA) with 15N,13C-labeling of either the N-terminal domain (NTD) or the C-terminal domain (CTD), and with high yield. CA assemblies formed in vitro from segmentally labeled products have greatly simplified 2D and 3D solid state NMR spectra, due to alleviation of spectral congestion. Segmentally labeled assemblies will be used to characterize intermolecular NTD-CTD interfaces and other local structural details within the mature HIV-1 capsid lattice. A paper describing these results is in preparation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIADK075032-09
Application #
9565925
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
9
Fiscal Year
2017
Total Cost
Indirect Cost

  Institution

Name
U.S. National Inst Diabetes/Digst/Kidney
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Gupta, Sebanti; Tycko, Robert (2018) Segmental isotopic labeling of HIV-1 capsid protein assemblies for solid state NMR. J Biomol NMR 70:103-114
Bayro, Marvin J; Tycko, Robert (2016) Structure of the Dimerization Interface in the Mature HIV-1 Capsid Protein Lattice from Solid State NMR of Tubular Assemblies. J Am Chem Soc 138:8538-46
Lu, Jun-Xia; Bayro, Marvin J; Tycko, Robert (2016) Major Variations in HIV-1 Capsid Assembly Morphologies Involve Minor Variations in Molecular Structures of Structurally Ordered Protein Segments. J Biol Chem 291:13098-112
Bayro, Marvin J; Ganser-Pornillos, Barbie K; Zadrozny, Kaneil K et al. (2016) Helical Conformation in the CA-SP1 Junction of the Immature HIV-1 Lattice Determined from Solid-State NMR of Virus-like Particles. J Am Chem Soc 138:12029-32
Bayro, Marvin J; Chen, Bo; Yau, Wai-Ming et al. (2014) Site-specific structural variations accompanying tubular assembly of the HIV-1 capsid protein. J Mol Biol 426:1109-27
Chen, Bo; Tycko, Robert (2011) Simulated self-assembly of the HIV-1 capsid: protein shape and native contacts are sufficient for two-dimensional lattice formation. Biophys J 100:3035-44
Lu, Jun-Xia; Sharpe, Simon; Ghirlando, Rodolfo et al. (2010) Oligomerization state and supramolecular structure of the HIV-1 Vpu protein transmembrane segment in phospholipid bilayers. Protein Sci :
Chen, Bo; Tycko, Robert (2010) Structural and dynamical characterization of tubular HIV-1 capsid protein assemblies by solid state nuclear magnetic resonance and electron microscopy. Protein Sci 19:716-30