Abstract

In previous years we had disrupted the genes encoding the pertussis toxin sensitive G proteins Gi2, Gi1, Gi3 and Go. Conditional knockouts for Gi2 and Go were also generated. Double knockouts involving Gi2 and Go are lethal. We expect to learn from combining Gi2 with Gi3 and enhanced survival of Go KO mice by removing the floxed genes at various times after birth. Breeding programs have been set up to add cre recombinase under several specific promoters so as to remove the genes both generally in all issues or in specific cell types such as in dopaminergic neurons to remove Go or lymphocytes to remove Gi from Gi3 KO mice. Most phenotypic studies were done in collaboration with outside investigators, Progress in this project was low and unsatisfactory andwas discontinued between Dec 15, 2013 and March 31, 2014.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAES101643-12
Application #
8929779
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
12
Fiscal Year
2014
Total Cost
Indirect Cost

  Institution

Name
U.S. National Inst of Environ Hlth Scis
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Liao, Yanhong; Abramowitz, Joel; Birnbaumer, Lutz (2014) The TRPC family of TRP channels: roles inferred (mostly) from knockout mice and relationship to ORAI proteins. Handb Exp Pharmacol 223:1055-75
Minetti, Giulia C; Feige, Jerome N; Bombard, Florian et al. (2014) G?i2 signaling is required for skeletal muscle growth, regeneration, and satellite cell proliferation and differentiation. Mol Cell Biol 34:619-30
Seo, Kinya; Rainer, Peter P; Shalkey Hahn, Virginia et al. (2014) Combined TRPC3 and TRPC6 blockade by selective small-molecule or genetic deletion inhibits pathological cardiac hypertrophy. Proc Natl Acad Sci U S A 111:1551-6
Tano, Jean-Yves; Solanki, Sumeet; Lee, Robert H et al. (2014) Bone marrow deficiency of TRPC3 channel reduces early lesion burden and necrotic core of advanced plaques in a mouse model of atherosclerosis. Cardiovasc Res 101:138-44
Oboti, Livio; Pérez-Gómez, Anabel; Keller, Matthieu et al. (2014) A wide range of pheromone-stimulated sexual and reproductive behaviors in female mice depend on G protein G?o. BMC Biol 12:31
Ball, Christopher B; Rodriguez, Karina F; Stumpo, Deborah J et al. (2014) The RNA-binding protein, ZFP36L2, influences ovulation and oocyte maturation. PLoS One 9:e97324
Kochukov, Mikhail Y; Balasubramanian, Adithya; Abramowitz, Joel et al. (2014) Activation of endothelial transient receptor potential C3 channel is required for small conductance calcium-activated potassium channel activation and sustained endothelial hyperpolarization and vasodilation of cerebral artery. J Am Heart Assoc 3:
Köhler, David; Devanathan, Vasudharani; Bernardo de Oliveira Franz, Claudia et al. (2014) G?i2- and G?i3-deficient mice display opposite severity of myocardial ischemia reperfusion injury. PLoS One 9:e98325
Choi, Chan-Il; Yoon, Sang-Phil; Choi, Jung-Mi et al. (2014) Simultaneous deletion of floxed genes mediated by CaMKII?-Cre in the brain and in male germ cells: application to conditional and conventional disruption of Go?. Exp Mol Med 46:e93
Ilatovskaya, Daria V; Palygin, Oleg; Chubinskiy-Nadezhdin, Vladislav et al. (2014) Angiotensin II has acute effects on TRPC6 channels in podocytes of freshly isolated glomeruli. Kidney Int 86:506-14

Showing the most recent 10 out of 46 publications