Abstract

The Arf6 GTP-binding protein has been shown to function at the plasma membrane to coordinate an endosomal membrane trafficking pathway and maintain the cortical actin cytoskeleton. We continue to follow functions for Arf6 as well as other Arf proteins at the cell surface and on endosomal membranes. We have also begun to examine functions of Arfs in other cells types in particular T cells. We continue to be interested in the issue of Arf proteins coordinating function with Rab proteins. In particular that Arf6 and Rab35 are antagonistic to each other in recruiting each other's GAPs. Thus activation of Arf6 leads to less activation of Rab35 and active Rab35 diminishes Arf6 activation. The cell uses this regulation to facilitate hand-off of cargo during endosomal trafficking.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAHL000517-21
Application #
9354305
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
21
Fiscal Year
2016
Total Cost
Indirect Cost

  Institution

Name
U.S. National Heart Lung and Blood Inst
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Donaldson, Julie G; Johnson, Debra L; Dutta, Dipannita (2016) Rab and Arf G Proteins in Endosomal Trafficking & Cell Surface Homeostasis. Small GTPases :0
Dutta, Dipannita; Donaldson, Julie G (2015) Rab and Arf G proteins in endosomal trafficking. Methods Cell Biol 130:127-38
Caviston, Juliane P; Cohen, Lee Ann; Donaldson, Julie G (2014) Arf1 and Arf6 promote ventral actin structures formed by acute activation of protein kinase C and Src. Cytoskeleton (Hoboken) 71:380-94
Donaldson, Julie G; Jackson, Catherine L (2011) ARF family G proteins and their regulators: roles in membrane transport, development and disease. Nat Rev Mol Cell Biol 12:362-75
Sakurai, Atsuko; Jian, Xiaoying; Lee, Charity J et al. (2011) Phosphatidylinositol-4-phosphate 5-kinase and GEP100/Brag2 protein mediate antiangiogenic signaling by semaphorin 3E-plexin-D1 through Arf6 protein. J Biol Chem 286:34335-45
Cohen, Lee Ann; Donaldson, Julie G (2010) Analysis of Arf GTP-binding protein function in cells. Curr Protoc Cell Biol Chapter 3:Unit 14.12.1-17
Grant, Barth D; Donaldson, Julie G (2009) Pathways and mechanisms of endocytic recycling. Nat Rev Mol Cell Biol 10:597-608
Scarselli, Marco; Donaldson, Julie G (2009) Constitutive internalization of G protein-coupled receptors and G proteins via clathrin-independent endocytosis. J Biol Chem 284:3577-85
Donaldson, Julie G; Williams, David B (2009) Intracellular assembly and trafficking of MHC class I molecules. Traffic 10:1745-52
Eyster, Craig A; Higginson, Jason D; Huebner, Robert et al. (2009) Discovery of new cargo proteins that enter cells through clathrin-independent endocytosis. Traffic 10:590-9

Showing the most recent 10 out of 14 publications