Abstract

A new spiral column assembly for analytical separation by counter-current chromatography is described. The column is made from a plastic spiral tube support which has 12 interwoven spiral grooves. The PTFE tubing of 1.6 mm ID was first flattened by extruding through a narrow slit and inserted into the grooves to make 5 spiral layers with about 60 ml capacity. The performance of the spiral column assembly was tested with separation of three stable protein samples including cytochrome C, myoglobin and lysozyme in a polymer phase system composed of polyethylene glycol 1000 and dibasic potassium phosphate each at 12.5 % (w/w) in water. At 2 ml/min, three protein samples were well resolved in one hour. The separation time may be further shortened by application of higher revolution speed and flow rate by improving the strength of the spiral tube support in the future.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAHL005107-08
Application #
9157351
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
2015
Total Cost
Indirect Cost

  Institution

Name
U.S. National Heart Lung and Blood Inst
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Zhang, Xinxin; Guo, Zengjun; Li, Jing et al. (2016) A new quantitation method of protodioscin by HPLC-ESI-MS/MS in rat plasma and its application to the pharmacokinetic study. Steroids 106:62-9
Shiono, Hiroyuki; Matsui, Takuya; Okada, Tadashi et al. (2016) Single-step enrichment of basophils from human peripheral blood by a novel method using a Percoll density gradient. J Sep Sci 39:3062-71
Zhang, Xinxin; Xue, Xuanji; Zhao, Jing et al. (2016) Quantitative determination of gracillin by HPLC-MS/MS after oral administration and its application to a pharmacokinetic study. Steroids 113:78-86
Zhang, Xinxin; Xue, Xuanji; Zhao, Jing et al. (2016) Diosgenin attenuates the brain injury induced by transient focal cerebral ischemia-reperfusion in rats. Steroids 113:103-12
Zhang, Xinxin; Xue, Xuanji; Xian, Liang et al. (2016) Potential neuroprotection of protodioscin against cerebral ischemia-reperfusion injury in rats through intervening inflammation and apoptosis. Steroids 113:52-63
Miyoshi, N; Kundu, S K; Tuziuti, T et al. (2016) Combination of Sonodynamic and Photodynamic Therapy against Cancer Would Be Effective through Using a Regulated Size of Nanoparticles. Nanosci Nanoeng 4:1-11
Zhang, Xinxin; Liang, Jinru; Zhang, Yongmin et al. (2015) Comparative studies on performance of CCC and preparative RP-HPLC in separation and purification of steroid saponins from Dioscorea zingiberensis C.H.Wright. J Steroids Horm Sci 6:
Dasarathy, Dhweeja; Ito, Yoichiro (2015) An improved design of spiral tube assembly for separation of proteins by high-speed counter-current chromatography. J Chromatogr A 1418:77-82
Liu, Yating; Tian, Ailin; Wang, Xiong et al. (2015) Fabrication of chiral amino acid ionic liquid modified magnetic multifunctional nanospheres for centrifugal chiral chromatography separation of racemates. J Chromatogr A 1400:40-6
Zhang, Min; Khripin, Constantine Y; Fagan, Jeffrey A et al. (2014) Single-step total fractionation of single-wall carbon nanotubes by countercurrent chromatography. Anal Chem 86:3980-4

Showing the most recent 10 out of 55 publications