Primary high-throughput screening was previously completed by the project team; during this period, the team has worked to validate and characterize hit compounds of interest. Compounds representing a number of distinct chemotypes were tested to determine their ability to enhance phagocytosis in primary human macrophages. Additionally, real-time monitoring of phagocytosis of live e-coli with macrophages overexpressing GPR32 treated with selected compounds was performed to confirm on-target activity. Binding modeling analysis was additionally conducted to predict docking sites and compare potential binding to that of RvD1.

Project Start
Project End
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Budget End
Support Year
5
Fiscal Year
2019
Total Cost
Indirect Cost
Name
National Center for Advancing Translational Sciences
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