Abstract

The Biotechnology Lab continued its collaborating work with the LDMI of the NICHD on the development of new vaccines against Shigella flexneri, Shigella Sonnie, Bortedella pertussis Haemophilus influenzae type A, Bacillus anthracis, influenza virus and malaria. Several proteins are being prepared for their evaluation as a potential candidates for conjugated polysaccharides vaccines, among them recombinant toxin A and toxin B of Clostridium difficle and recombinant diphtheria toxoid, recombinant pfs25H and pvs25H are being made for experimental malaria transmission blocking vaccine. The Biotechnology Lab continued its collaboration with the Malaria Vaccine Development Unit of NIAID working on improving the production of various experimental malaria vaccines. Working together with scientists from the FDA the laboratory is evaluating the potential production of influenza virus vaccine form the newly developed MDCK cells, and on the structure of the polysaccharide from E. coli K1 and Meningococcal C.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICDK070011-06
Application #
8554137
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
2012
Total Cost
$315,595
Indirect Cost

  Institution

Name
National Institute of Diabetes and Digestive and Kidney Diseases
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Robbins, John B; Schneerson, Rachel; Kubler-Kielb, Joanna et al. (2014) Toward a new vaccine for pertussis. Proc Natl Acad Sci U S A 111:3213-6
Battistel, Marcos D; Shangold, Michael; Trinh, Loc et al. (2012) Evidence for helical structure in a tetramer of ?2-8 sialic acid: unveiling a structural antigen. J Am Chem Soc 134:10717-20
Bellanti, Joseph A; Lin, Feng-Ying C; Chu, Chiayung et al. (2012) Phase 1 study of a recombinant mutant protective antigen of Bacillus anthracis. Clin Vaccine Immunol 19:140-5
Cui, Xizhong; Su, Junwu; Li, Yan et al. (2010) Bacillus anthracis cell wall produces injurious inflammation but paradoxically decreases the lethality of anthrax lethal toxin in a rat model. Intensive Care Med 36:148-56
Chu, Chia; Lugovtsev, Vladimir; Lewis, Andrew et al. (2010) Production and antigenic properties of influenza virus from suspension MDCK-siat7e cells in a bench-scale bioreactor. Vaccine :
Passwell, Justen H; Ashkenzi, Shai; Banet-Levi, Yonit et al. (2010) Age-related efficacy of Shigella O-specific polysaccharide conjugates in 1-4-year-old Israeli children. Vaccine 28:2231-5
Robbins, John B; Kubler-Kielb, Joanna; Vinogradov, Evguenii et al. (2009) Synthesis, characterization, and immunogenicity in mice of Shigella sonnei O-specific oligosaccharide-core-protein conjugates. Proc Natl Acad Sci U S A 106:7974-8
Biesova, Zuzana; Miller, Mark A; Schneerson, Rachel et al. (2009) Preparation, characterization, and immunogenicity in mice of a recombinant influenza H5 hemagglutinin vaccine against the avian H5N1 A/Vietnam/1203/2004 influenza virus. Vaccine 27:6234-8