The Chromosome Pathology Unit (CPU)was opened in 2010 to fulfill the need in the """"""""in house"""""""" laboratory FISH tests required to support clinical trials at NCI. The official CAP accreditation and CLIA certification has been accomplished by the CPU team in November 22, 2010. From January 2011 the Lab started receiving clinical cases for FISH testing. The CPU performed more than 2,600 tests since its opening. This includes FISH assays for over 1120 clinical reported tests, over 5000 tests for probe validation and collaboration with LP and other NIH researchers. FISH diagnostics are an integral part of the Pathology molecular diagnostics currently offered by the Lab of Pathology. The sensitivity and specificity of FISH diagnostics was found to be near 100% in recent reports of CAP that puts them in front line among other available tests based on PCR and immunohistochemistry that have a long-known specificity problem. The Chromosome Pathology Unit (CPU) in the Laboratory of Pathology performs Fluorescence In Situ Hybridization (FISH) assays on formalin-fixed paraffin-embedded (FFPE) tissues to support active clinical trials at the NCI and NIH. The demand for this testing is high;the CPU receives orders for FISH tests on clinical tumor samples daily, and currently supports 17 clinical trials at the NCI. That includes requests from the entire community of CCR investigators: Molecular Oncology Branch, Surgery Branch, Office of the Director, Pediatric Oncology Branch, Neuro-Oncology Branch, Urologic Oncology Branch. The active test menu includes FISH assays for clinically significant chromosomal translocations and amplification events in solid tumors and hematologic malignancies. The developed/ validated tests include Her-2 amplification in Breast, Lung, and GI tract cancer, c-myc translocation in Burkitt and Diffuse Large B Cell Lymphoma, BCL2 translocation in Follicular Lymphoma, 1p/19q deletion tests for brain tumors, ALK translocation in lung cancer and inflammatory myofibroblastic tumors, FGFR1, PDGFRA and PIK3A amplification in lung cancer, NTRK1 amplification in thymic tumors, TFE-3 translocation in renal tumors, alveolar soft part sarcoma, EWSR1/FLI1 and FKHR translocations in pediatric tumors (Ewing's sarcoma and Rabdomyosarcoma), etc. In addition, the CPU is currently developing and validating ten new FISH tests for additional frequent chromosomal abnormalities in cancers that have been requested by NCI investigators. Among the tests in the pipeline are RET rearrangements in thyroid cancer, ERG translocation in prostate cancer, TFE-B gene translocation in renal tumors (responsible for 30% of pediatric kidney cancer). The CPU has been working on developing novel diagnostics that would significantly advance currently existing approaches in cancer clinical testing. One of them is Isolation and Molecular Profiling of the Circulating Tumor Cells (CTCs)from the peripheral blood of cancer patients as an alternative non-invasive biopsy method for detection and monitoring tumor metastatic disease and treatment efficiency. The Unit has been equipped as state-of-the art laboratory that includes a new automated Fluorescence imaging workstation Duet from BioView. Inc, Zeiss epifluorescence microscope with the ApoTome feature that allows FISH analysis and imaging of thick tissue sections, SKY-cube for spectral karyotyping - a modern tool for the detection of chromosomal translocations in cancer cells and various hereditary syndromes.
| Arnold, Michael A; Ballester, Leomar Y; Pack, Svetlana D et al. (2014) Primary subcutaneous spindle cell Ewing sarcoma with strong S100 expression and EWSR1-FLI1 fusion: a case report. Pediatr Dev Pathol 17:302-7 |
| Zhuang, Zhengping; Frerich, Jason M; Huntoon, Kristin et al. (2014) Tumor derived vasculogenesis in von Hippel-Lindau disease-associated tumors. Sci Rep 4:4102 |
| Mardekian, Stacey K; Gandhe, Ashish; Miettinen, Markku et al. (2014) Two Cases of Spinal, Extraosseous, Intradural Ewing's sarcoma/Peripheral Neuroectodermal Tumor: Radiologic, Pathologic, and Molecular Analysis. J Clin Imaging Sci 4:6 |
| Zhang, Chao; Yang, Andrew I; Vasconcelos, Lucas et al. (2014) Von hippel-lindau disease associated pulmonary carcinoid with cranial metastasis. J Clin Endocrinol Metab 99:2633-6 |
| Sadri, Navid; Barroeta, Julieta; Pack, Svetlana D et al. (2014) Malignant round cell tumor of bone with EWSR1-NFATC2 gene fusion. Virchows Arch 465:233-9 |
| Mamessier, Emilie; Song, Joo Y; Eberle, Franziska C et al. (2014) Early lesions of follicular lymphoma: a genetic perspective. Haematologica 99:481-8 |
| Nicolae, A; Xi, L; Pittaluga, S et al. (2014) Frequent STAT5B mutations in ?? hepatosplenic T-cell lymphomas. Leukemia 28:2244-8 |
| Li, Hongzhen; Rodriguez-Canales, Jaime; Liu, Wenli et al. (2013) Deletion of the olfactomedin 4 gene is associated with progression of human prostate cancer. Am J Pathol 183:1329-38 |
| Miettinen, Markku; Wang, Zengfeng; Sarlomo-Rikala, Maarit et al. (2013) ERG expression in epithelioid sarcoma: a diagnostic pitfall. Am J Surg Pathol 37:1580-5 |
| Aung, Phyu P; Ballester, Leomar Y; Abdullaev, Zied et al. (2013) ER/PR positive epidermotropic primary cutaneous eccrine carcinoma as a cutaneous manifestation of MEN 2B. J Am Acad Dermatol 69:e310-2 |
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