The Transgenic Mouse Model (TMM) Laboratory at the National Cancer Institute ? Frederick provides assistance to investigators with a need to study in vivo gene function, by producing transgenic mice by pronuclear microinjection of fertilized mouse eggs. Although this service is customized to the needs of each client, the core of this effort includes purification of the DNA fragment to be microinjected and analysis of the resulting mice by Southern blot, PCR, or slot/dot blot. Consultations for the design of recombinant DNA constructs for the use in transgenic mouse studies are available. Characterization of the pattern of transgene expression at the RNA or protein level is also available. Transgenic mice are routinely produced in FVB/N and C57BL/6 backgrounds.

Agency
National Institute of Health (NIH)
Type
Research and Development Contracts (N01)
Project #
261200800001E-52-0-32
Application #
8654771
Study Section
Project Start
2008-09-26
Project End
2018-09-25
Budget Start
Budget End
Support Year
Fiscal Year
2013
Total Cost
$590,309
Indirect Cost
Name
Saic-Frederick, Inc.
Department
Type
DUNS #
159990456
City
Frederick
State
MD
Country
United States
Zip Code
21702