Pseudomonas aeruginosa is an important nosocomial pathogen, not only because it causes a significant amount of morbidity and mortality, but also because it develops significant clinical resistance to many antibiotics, presenting the threat of even greater morbidity and mortality. We will use parallel genetic screens utilizing DNA oligonucleotide arrays to identify genes and the interactions between genes in P. aeruginosa that are involved in its resistance to the aminoglycoside: amikacin. Techniques utilized will include parallel gene trait mapping (PGTM), insertional mutagenesis, and a bacterial two-hybrid screen. The information obtained regarding identified genes and there interactions will be used to identify possible drug targets that have a greater impact on amikacin resistance as well as offering drug specificity. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31AI056687-02
Application #
6779864
Study Section
Special Emphasis Panel (ZRG1-F08 (20))
Program Officer
Hernandez, Milton J
Project Start
2003-07-01
Project End
2006-06-30
Budget Start
2004-07-01
Budget End
2005-06-30
Support Year
2
Fiscal Year
2004
Total Cost
$30,048
Indirect Cost
Name
University of Colorado at Boulder
Department
Engineering (All Types)
Type
Schools of Engineering
DUNS #
007431505
City
Boulder
State
CO
Country
United States
Zip Code
80309