The ability of bacteria to survive antibiotic treatment remains a significant challenge for controlling bacterial infections worldwide. Unlike drug resistance where a mutation must evolve and be selected for, all microbial populations harbor "persister cells" which are a non-growing cell subpopulation highly tolerant to antibiotic action. These persister cells are a major cause of the multidrug tolerance of chronic infectious pathogens including tuberculosis (TB), the leading cause of bacterial mortality worldwide, as well as P. aeruginosa, and S. aureus. While much attention has been paid to antibiotic resistance, the mechanisms of antibiotic tolerance remain poorly understood despite their obvious importance for human health. We have identified ribosomal hibernation as a process that contributes to antibiotic tolerance in Listeria monocytogenes (Lm). During stress, Hibernation Promotion Factor (HPF), a conserved ribosome associated protein, is expressed and mediates dimerization of two 70S ribosomes into a translationally silent "hibernating" 100S ribosome. 100S ribosomes have been hypothesized to globally downregulate translation and promote "stasis" during stress, however, neither role has been experimentally demonstrated since their discovery nearly 30 years ago. Our work has demonstrated that 100S ribosomes are induced in response to treatment with aminoglycoside antibiotics and that deletion of HPF abolishes 100S ribosome formation and renders cells sensitive to these drugs. We hypothesize that ribosomal hibernation mediated by HPF alters translation in ways that contribute to antibiotic tolerance. The objective of this proposal is to characterize the role of ribosomal hibernation in antibiotic tolerance and to define the molecular mechanisms by which HPF-mediated 100S ribosomes facilitate persistence in Lm.
In Aim1, we propose to use genome-wide analysis of in vivo translation to identify the genes and biological pathways regulated in response to ribosomal hibernation.
In Aim 2, we will evaluate the mechanism(s) by which HPF-mediated "hibernating" ribosomes facilitate adaptive responses and promote antibiotic tolerance. These studies will be the first to describe a role for 100S ribosomes in antibiotic tolerance and lead to a better understanding of the mechanisms underpinning persistence. Since HPF is conserved in nearly every bacterial pathogen including those responsible for chronic infections like TB, understanding the role of HPF-mediated ribosomal hibernation in bacterial persistence has broad implications for human health.

Public Health Relevance

Understanding how bacterial pathogens survive antibiotic treatment will improve our ability to control disease. The proposed research seeks to understand how the process of ribosomal hibernation contributes to antibiotic tolerance by regulating protein translation in the foodborne pathogen Listeria monocytogenes. Since the ability to form hibernating ribosomes is conserved among many bacteria, we expect that our findings will be broadly applicable for those studying bacterial pathogenesis in other species, particularly the causative agents of chronic infections such as Mycobacterium tuberculosis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32AI108253-01
Application #
8596596
Study Section
Special Emphasis Panel (ZRG1-F13-C (20))
Program Officer
Mills, Melody
Project Start
2013-06-01
Project End
2013-08-31
Budget Start
2013-06-01
Budget End
2013-08-31
Support Year
1
Fiscal Year
2013
Total Cost
$67,200
Indirect Cost
Name
University of California Berkeley
Department
Biochemistry
Type
Other Domestic Higher Education
DUNS #
124726725
City
Berkeley
State
CA
Country
United States
Zip Code
94704