This research focuses on the discovery of genetic and developmental mechanisms that govern evolutionary change in embryogenesis and early larval development. By using a non-model system with a heritable developmental dimorphism, I can directly ascertain key mechanistic changes in early development and oogenesis within a single species. This approach eliminates the potentially confounding effects of more traditional comparative approaches, and has applications for genetic diagnosis of fertilization competent eggs. Here I propose to use the developmental dimorphism in the marine polychaete Streblospio benedicti to characterize the extent of genomic divergence contributing to egg size and larval development. Using quantitative genomics and comparative transcriptomics, I will determine the specific loci involved in regulating this development mode determination, and quantify the extent that these variants act maternally, zygotically or both. Specifically, I will ue linkage mapping to identify quantitative trait loci (QTLs) associated with developmental and reproductive traits. In addition, I will use the transcriptomes of oocytes and early embryos to ascertain how these two offspring types differ in abundance and timing of gene expression. The proposed research is the first study to investigate how critical developmental determinants such as egg size can evolve using a single species.

Public Health Relevance

The proposed research has the potential to improve human health by greatly increasing our understanding of developmental evolution and constraint by identifying genomic regions involved in oogenesis and early development. We can gain valuable insight into the genetic architecture underlying variation in early development and how this ultimately translates to variation in fitness. By studying the regulation of oogenesis in particula, we can contribute to genetic diagnosis of fertilization competent eggs and infertility.

Agency
National Institute of Health (NIH)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32GM108396-01A1
Application #
8783773
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Reddy, Michael K
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost
Name
New York University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10012