Because of the numerous functions they perform during morphogenesis, much of the understanding of TGFB signal transduction stems from studies in developing embryos. However, as these observations rely primarily on examination of TGFI3 ligands and their receptors, a more complete understanding of their role in embryogenesis requires direct observation of when and where TGFB signals are activated. To accomplish this, an antibody has been generated recognizing the active, phosphorylated form of Smads 1, 5, and 8, which transduces signaling by bone morphogenetic proteins (BMPs), a subset of the TGFBs. This antibody will be used to characterize the pattern of Smad/l5/8 phosphorylation in post-gastrula Xenopus embryos. In addition, methods for conditional gene expression will be validated. These will be used to conditionally activate and inhibit endogenous BMP signaling in order examine functional requirements for BMPs after gastrulation. Finally, preliminary observations reveal a domain of Srnadll5/8 phosphorylation in presumptive neural crest cells. Experiments are proposed to address mechanisms by which domains of BMP signaling are established during neural crest specification and functional requirements for BMPs at specific times during neural crest development.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32HD041313-02
Application #
6526570
Study Section
Cell Development and Function Integrated Review Group (CDF)
Program Officer
Klein, Steven
Project Start
2002-04-01
Project End
Budget Start
2003-04-01
Budget End
2004-03-31
Support Year
2
Fiscal Year
2003
Total Cost
$41,608
Indirect Cost
Name
Harvard University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
047006379
City
Boston
State
MA
Country
United States
Zip Code
02115