The PI is developing a "molecular microscope" capable of taking nanometer-resolution magnetic resonance images of individual macromolecules. In support of this work, the PI will be trained in state-of-the- art cryo-electron microscopy, and will carry out research to develop improved approaches for preparing fragile biomacromolecules for study at low temperatures in vacuum. The proposed research in sample preparation will make it much easier to study a wider range of samples by both cryo-electron microscopy and nanometer-resolution magnetic resonance imaging.

Public Health Relevance

In order to better understand the functioning of both diseased and healthy tissue, new tools for deter- mining the three dimensional structure of single macromolecules and macromolecular complexes are urgently needed. This grant will improve our ability to use cryo-electron microscopy to study precious or difficult-to-prepare samples such as membrane proteins. It will also accelerate development of nanometer-resolution magnetic resonance imaging.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
National Research Service Awards for Senior Fellows (F33)
Project #
1F33GM105241-01
Application #
8457701
Study Section
Special Emphasis Panel (ZRG1-F04-D (20))
Program Officer
Flicker, Paula F
Project Start
2013-01-07
Project End
2013-08-15
Budget Start
2013-01-07
Budget End
2013-08-15
Support Year
1
Fiscal Year
2013
Total Cost
$40,620
Indirect Cost
Name
Scripps Research Institute
Department
Type
DUNS #
781613492
City
La Jolla
State
CA
Country
United States
Zip Code
92037