This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. To assess the effects of vitamin D on the expression of pro inflammatory cytokines in human myometrial cells. In African Americans the incidence of preterm birth (PTB) is two times higher compared with that in other ethnicities. In addition they have low serum levels of vitamin D. We believe that vitamin D deficiency as observed in Blacks increase inflammatory proteins. Therefore increase in inflammation during pregnancy before term can be risk factor for preterm birth. However, studies on the role of Vitamin D in the regulation of inflammatory proteins in myometrium are lacking. Objective: To investigate the role of vitamin D in the regulation of various inflammatory markers and related receptors in immortalized myometrial (UtSM) cells. Results: Human cytokine and receptor super array performed in RNA obtained from UtSMC treated with Vitamin D showed down regulation of IL9, IL10, IL12B, IL13, CCL2, CCL13, CXCL10, CX3CL1, CSF2 and receptors IL1RN and IL2RA.and up regulation of markers , CXCR4, CCR4 and receptors IL7R, IL23R and LIFR and RT-PCR analysis showed significant decreases (p0.05) in the expression of MCP-1, FPR and Connexin-43.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Research Centers in Minority Institutions Award (G12)
Project #
5G12RR003032-27
Application #
8357138
Study Section
Special Emphasis Panel (ZRR1-RI-B (01))
Project Start
2011-07-01
Project End
2012-06-30
Budget Start
2011-07-01
Budget End
2012-06-30
Support Year
27
Fiscal Year
2011
Total Cost
$134,776
Indirect Cost
Name
Meharry Medical College
Department
Type
Schools of Medicine
DUNS #
041438185
City
Nashville
State
TN
Country
United States
Zip Code
37208
Al-Hendy, Ayman; Laknaur, Archana; Diamond, Michael P et al. (2017) Silencing Med12 Gene Reduces Proliferation of Human Leiomyoma Cells Mediated via Wnt/?-Catenin Signaling Pathway. Endocrinology 158:592-603
Morris, Matthew C; Abelson, James L; Mielock, Alyssa S et al. (2017) Psychobiology of cumulative trauma: hair cortisol as a risk marker for stress exposure in women. Stress 20:350-354
Morris, Matthew C; Kouros, Chrystyna D; Janecek, Kim et al. (2017) Community-level moderators of a school-based childhood sexual assault prevention program. Child Abuse Negl 63:295-306
Laknaur, Archana; Foster, Terri-Lee; Bobb, Lesley E et al. (2016) Altered expression of histone deacetylases, inflammatory cytokines and contractile-associated factors in uterine myometrium of Long Evans rats gestationally exposed to benzo[a]pyrene. J Appl Toxicol 36:827-35
Al-Hendy, Ayman; Diamond, Michael P; Boyer, Thomas G et al. (2016) Vitamin D3 Inhibits Wnt/?-Catenin and mTOR Signaling Pathways in Human Uterine Fibroid Cells. J Clin Endocrinol Metab 101:1542-51
Morris, Matthew C; Walker, Lynn S; Bruehl, Stephen et al. (2016) Impaired conditioned pain modulation in youth with functional abdominal pain. Pain 157:2375-81
Clark, Ryan S; Pellom, Samuel T; Booker, Burthia et al. (2016) Validation of research trajectory 1 of an Exposome framework: Exposure to benzo(a)pyrene confers enhanced susceptibility to bacterial infection. Environ Res 146:173-84
Wilson, Andrew J; Fadare, Oluwole; Beeghly-Fadiel, Alicia et al. (2015) Aberrant over-expression of COX-1 intersects multiple pro-tumorigenic pathways in high-grade serous ovarian cancer. Oncotarget 6:21353-68
Halder, Sunil K; Laknaur, Archana; Miller, Jessica et al. (2015) Novel MED12 gene somatic mutations in women from the Southern United States with symptomatic uterine fibroids. Mol Genet Genomics 290:505-11
Morris, Matthew C; Evans, Lindsay D; Rao, Uma et al. (2015) Executive function moderates the relation between coping and depressive symptoms. Anxiety Stress Coping 28:31-49

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