The fundamental goals of this research proposal are to test the hypotheses that: 1) application of thermal energy at 65 degrees C using radio-frequency energy to the coxofemoral joint capsule of puppies with radiographic evidence of joint laxity will result in normal hip congruity, 2) will prevent the development of the lameness and the cartilagenous and bony changes characteristic of CHD, and 3) will not have detrimental effects on the biomechanical or viscoelastic properties of the joint capsular tissue. These investigations will yield insights into the fundamental mechanisms of thermal modification of musculoskeletal soft tissue. This research program is designed to determine whether thermal modification of coxocofemoral joint capsule of 16-week-old puppies with excessive joint: laxity will prevent CHD. Ultimately, this model will serve to evaluate the potential of thermal energy to reduce excessively lax coxofemoral joints in neonatal children and thereby help prevent DDH. The candidate of this application graduated magna cum laude from Humboldt State University with a B.S. in cellular-molecular biology and a minor in chemistry. She worked as a molecular biologist at Amgen, Thousand Oaks, CA, before undertaking and; completing her D.V.M. at the University of California-Davis. Dr. Lopez was awarded 5 scholarships and 2 research grants while a D.V.M. student. She completed an internship at Kansas State University and a large animal surgery residency/Master's degree at they University of Wisconsin- Madison. She is a board certified large animal surgeon. Dr. Lopez's Master's thesis was entitled """"""""Effects of Radio-frequency Energy on Ovine Stifle Joint Capsule"""""""", and she performed much of the early radio- frequency work in the Comparative Orthopaedic Research Laboratory. She was awarded an NRSA in 1998 and has performed the background research and preliminary, studies that form the basis for this proposal. She passed her preliminary examinations """"""""With Honors"""""""" in November, 1999, and is scheduled to complete her PhD in June 2001. Her ultimate goal is to join the faculty of an academic institution as a well-trained scientist and productive researcher specializing in integrative, translational research. The training environment will be guided toward mentoring the candidate while teaching valuable research techniques necessary to begin an independent research career. The majority of supervision will be provided by Mark D. Markel, an internationally known and respected investigator in the area of thermal tissue modification, Professor of Surgery, and Professor and Chairman of Medical Sciences. The advisory committee will consist of well-respected and highly experienced individuals within the University. The Comparative Orthopaedic Research Laboratory is a well established laboratory that houses multi- disciplinary research programs. The University of Wisconsin provides an exceptional environment for interaction with experts in a variety of fields, and it is well known for its exceptional research programs, especially in the medical sciences.

National Institute of Health (NIH)
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Research Scientist Development Award - Research & Training (K01)
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Study Section
Arthritis and Musculoskeletal and Skin Diseases Special Grants Review Committee (AMS)
Program Officer
Panagis, James S
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University of Wisconsin Madison
Other Basic Sciences
Schools of Veterinary Medicine
United States
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Andronescu, Anemone A; Kelly, Laura; Kearney, Michael T et al. (2015) Associations between early radiographic and computed tomographic measures and canine hip joint osteoarthritis at maturity. Am J Vet Res 76:19-27
Spencer, Nakia D; Chun, Raymond; Vidal, Martin A et al. (2012) In vitro expansion and differentiation of fresh and revitalized adult canine bone marrow-derived and adipose tissue-derived stromal cells. Vet J 191:231-9
D'Amico, Laura L; Xie, Lin; Abell, Lindsey K et al. (2011) Relationships of hip joint volume ratios with degrees of joint laxity and degenerative disease from youth to maturity in a canine population predisposed to hip joint osteoarthritis. Am J Vet Res 72:376-83
Spencer, Nakia D; Lopez, Mandi J (2011) In vitro adult canine adipose tissue-derived stromal cell growth characteristics. Methods Mol Biol 702:47-60
Lopez, Mandi J; Davis, Kechia M; Jeffrey-Borger, Susan L et al. (2009) Interobserver repeatability of measurements on computed tomography images of lax canine hip joints from youth to maturity. Vet Surg 38:920-6
Vasanjee, Sunil C; Paulsen, Daniel; Hosgood, Giselle et al. (2008) Characterization of normal canine anterior cruciate ligament-associated synoviocytes. J Orthop Res 26:809-15
Lopez, Mandi J; Spencer, Nakia; Casey, John P et al. (2007) Biomechanical characteristics of an implant used to secure semitendinosus-gracilis tendon grafts in a canine model of extra-articular anterior cruciate ligament reconstruction. Vet Surg 36:599-604
Lopez, Mandi J; Quinn, Margaret M; Markel, Mark D (2006) Associations between canine juvenile weight gain and coxofemoral joint laxity at 16 weeks of age. Vet Surg 35:214-8
Lopez, Mandi J; Markel, Mark D (2006) Monopolar radiofrequency energy application to the dorsal extensor tendon apparatus in a canine model of tendon injury. J Hand Surg Am 31:1296-302
Lopez, Mandi J; Robinson, Sandra O; Quinn, Margaret M et al. (2006) In vivo evaluation of intra-articular protection in a novel model of canine cranial cruciate ligament mid-substance elongation injury. Vet Surg 35:711-20

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