Chronic exposure of the liver to elevated FAs, as occurs on a high-fat diet (HFD), promotes liver gluconeogenesis, reduces enzymes of FA oxidation and increases hepatic lipogenesis, leading to increased liver triglyceride content (i.e., fatty liver) and insulin resistance. This is especially the case as visceral obesity develops. While there is evidence that dysregulation of FA storage within adipose tissue provides a fundamental link among obesity, insulin resistance and type-2 diabetes (T2D), the contribution of fatty acid trafficking in visceral fat is often underemphasized. Based on our preliminary data, we have hypothesized that excess FA flux to the liver via the hepatic portal vein is a key factor, and that it can occur for different reasons. We believe that this situation can be ameliorated, at least temporarily, by increasing the capacity of visceral fat (i.e., mesenteric white adipose tissue or WAT) to sequester FA from the blood, thereby reducing flux to the liver. In support of this, we have evidence that increasing visceral adipose mass due to local adipocyte proliferation or transplantation of adipose tissue into the mesenteric region improves glucose tolerance;i.e., visceral adipose tissue mass per se is not a reliable predictor of insulin resistance. We propose to test the hypothesis that insulin resistance/glucose intolerance can be reversed by procedures that promote adipocyte expansion and FA retention in the visceral bed.
Specific Aims are: 1.) To test the hypothesis that DIO (HFD- induced obesity) increases hepatic insulin resistance in part due to insufficient removal of FA from blood by proliferating adipocytes. This will be accomplished by investigating small adipocyte growth-induced improvements in glucose tolerance via characterizing specific adipocyte cell stage and anatomic location in terms of the capacity to store and retain FA. We will also demonstrate if adipocyte proliferation induction is prevented, then concurrent improvement of glucose tolerance is prevented as well. Lastly, we will induce insulin resistance via a selective increase of portal FA influx to the liver. 2.) To test the hypothesis that the development of FA dysregulation in mature adipocytes is due to reduced insulin-mediated FA retention. We predict that transplantation-induced glycemic improvements result from adipose depot-specific differences in insulin-mediated actions within mature adipocytes, and that these in turn are related to the relative neural (i.e., sympathetic) activity in specific fat depots. We will compare the individual contribution of adrenergic-induced lipolytic effects and insulin FA uptake/anti-lipolytic effects on glucose tolerance in animals receiving different transplants. Collectively, these experiments will provide important new information on the regulation of liver functioning by FA released from adipocytes and/or diet. Further, the studies proposed have direct clinical relevance in that they may provide novel mechanisms as to how adipocyte dysregulation increases the risk of diabetes.

Public Health Relevance

Project Narrative: Chronic exposure of the liver to elevated FAs, as occurs on a high-fat diet (HFD), promotes liver gluconeogenesis, reduces enzymes of FA oxidation and increases hepatic lipogenesis, leading to increased liver triglyceride content (i.e., fatty liver) and insulin resistance. While there is evidence that dysregulation of FA storage within adipose tissue provides a fundamental link among obesity, insulin resistance and type-2 diabetes (T2D), the contribution of fatty acid trafficking in visceral fat is often underemphasized. The goal of this proposal is to determine the link between adipocyte expansion and FA retention in the visceral bed and insulin resistance/glucose intolerance.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Scientist Development Award - Research & Training (K01)
Project #
5K01DK087816-04
Application #
8220974
Study Section
Special Emphasis Panel (ZDK1-GRB-W (J3))
Program Officer
Hyde, James F
Project Start
2010-05-04
Project End
2014-03-31
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
4
Fiscal Year
2012
Total Cost
$150,055
Indirect Cost
$11,115
Name
Colorado State University-Fort Collins
Department
Nutrition
Type
Other Domestic Higher Education
DUNS #
785979618
City
Fort Collins
State
CO
Country
United States
Zip Code
80523
Lee, Dustin M; Battson, Micah L; Jarrell, Dillon K et al. (2017) Fuzhuan tea reverses arterial stiffening after modest weight gain in mice. Nutrition 33:266-270
Booth, A D; Magnuson, A M; Cox-York, K A et al. (2017) Inhibition of adipose tissue PPAR? prevents increased adipocyte expansion after lipectomy and exacerbates a glucose-intolerant phenotype. Cell Prolif 50:
Foster, Michelle T (2017) So as we worry we weigh: Visible burrow system stress and visceral adiposity. Physiol Behav 178:151-156
Booth, Andrea; Magnuson, Aaron; Fouts, Josephine et al. (2016) Adipose tissue: an endocrine organ playing a role in metabolic regulation. Horm Mol Biol Clin Investig 26:25-42
Foster, Michelle T; Gentile, Christopher L; Cox-York, Kimberly et al. (2016) Fuzhuan tea consumption imparts hepatoprotective effects and alters intestinal microbiota in high saturated fat diet-fed rats. Mol Nutr Food Res 60:1213-20
Cox-York, K; Wei, Y; Wang, D et al. (2015) Lower body adipose tissue removal decreases glucose tolerance and insulin sensitivity in mice with exposure to high fat diet. Adipocyte 4:32-43
Booth, Andrea; Magnuson, Aaron; Fouts, Josephine et al. (2015) Adipose tissue, obesity and adipokines: role in cancer promotion. Horm Mol Biol Clin Investig 21:57-74
Cox-York, Kimberly A; Sheflin, Amy M; Foster, Michelle T et al. (2015) Ovariectomy results in differential shifts in gut microbiota in low versus high aerobic capacity rats. Physiol Rep 3:
Gentile, C L; Weir, T L; Cox-York, K A et al. (2015) The role of visceral and subcutaneous adipose tissue fatty acid composition in liver pathophysiology associated with NAFLD. Adipocyte 4:101-12
Booth, Andrea; Magnuson, Aaron; Foster, Michelle (2014) Detrimental and protective fat: body fat distribution and its relation to metabolic disease. Horm Mol Biol Clin Investig 17:13-27

Showing the most recent 10 out of 17 publications