: Dr. Rose obtained his Ph.D. in molecular biology, studying SV40-induced cellular transformation. During his postdoctoral fellowship, his entire laboratory left academia to form a new biotechnology company. There, Dr. Rose set up molecular cloning and bioinformatics and focused his research on the identification of new growth factors. He also developed a technique to identify distantly related sequences using consensus-degenerate hybrid oligonucleotide primers (CODEHOPs). In 1995, Dr. Rose decided to leave the biotech industry and pursue an academic research career. Shortly after, he used CODEHOPs to discover three new viruses related to KSHV in RF. He developed the hypothesis that the macaque herpesviruses could play an etiological role in the pathogenesis of RF and that RF would be a relevant animal model of AIDS-KS. Dr. Rose has obtained R01 funding to characterize these viruses and is starting to develop a career in infectious diseases. Due to his previous focus in molecular biology, he lacks specific training in viral culture and biology of herpesvirus. His retraining and research efforts have been slowed by the need to pursue other aspects of his academic career for funding reasons, including teaching and program development. Salary support from the K02 award would free him from the current dependency on administrative and teaching duties during this critical turning point in his career and allow him to focus more closely on the characterization of the new viruses. Career development and training would also include participating in virology classes, seminars, conferences and meetings, and learning new techniques from experts in the field. His long-term goal is to determine the contributions and interactions of the new herpesviruses with retroviruses and endogenous cellular factors in the induction and progression of KS-like malignancies. His specific research aims are: 1) to determine the genomic organization of the macaque herpesvirus, RFHVMn; 2) to determine the cellular tropism and replicative state of the new macaque herpesviruses; and 3) to establish the baseline parameters of the infectious cycle of the macaque viruses. Additional training and focused research in virology would allow Dr. Rose to expand upon his initial discovery and make further significant contributions in the fields of herpesviruses and viral pathogenicity.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Scientist Development Award - Research (K02)
Project #
5K02AI049275-05
Application #
6862728
Study Section
Acquired Immunodeficiency Syndrome Research Review Committee (AIDS)
Program Officer
Brobst, Susan W
Project Start
2001-05-01
Project End
2006-04-30
Budget Start
2005-05-01
Budget End
2006-04-30
Support Year
5
Fiscal Year
2005
Total Cost
$98,658
Indirect Cost
Name
University of Washington
Department
Pathology
Type
Schools of Public Health
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
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Burnside, Kellie L; Ryan, Jonathan T; Bielefeldt-Ohmann, Helle et al. (2006) RFHVMn ORF73 is structurally related to the KSHV ORF73 latency-associated nuclear antigen (LANA) and is expressed in retroperitoneal fibromatosis (RF) tumor cells. Virology 354:103-15
Philipp-Staheli, Jeannette; Marquardt, Taya; Thouless, Margaret E et al. (2006) Genetic variability of the envelope gene of Type D simian retrovirus-2 (SRV-2) subtypes associated with SAIDS-related retroperitoneal fibromatosis in different macaque species. Virol J 3:11
Bruce, A Gregory; Bakke, Angela M; Bielefeldt-Ohmann, Helle et al. (2006) High levels of retroperitoneal fibromatosis (RF)-associated herpesvirus in RF lesions in macaques are associated with ORF73 LANA expression in spindleoid tumour cells. J Gen Virol 87:3529-38
Bruce, A Gregory; Bakke, Angela M; Thouless, Margaret E et al. (2005) Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons. Virol J 2:2
Bielefeldt-Ohmann, H; Barouch, D H; Bakke, A M et al. (2005) Intestinal stromal tumors in a simian immunodeficiency virus-infected, simian retrovirus-2 negative rhesus macaque (Macaca mulatta). Vet Pathol 42:391-6
Rose, Timothy M; Ryan, Jonathan T; Schultz, Emily R et al. (2003) Analysis of 4.3 kilobases of divergent locus B of macaque retroperitoneal fibromatosis-associated herpesvirus reveals a close similarity in gene sequence and genome organization to Kaposi's sarcoma-associated herpesvirus. J Virol 77:5084-97