The goal of this research is to develop a computational platform that simulates light transport in a system that considers explicitly the structural and compositional complexity of tissue. This virtual tissue simulator (VTS) for radiative transport will allow representations of tissues that are either user-defined using 'primitive1 tissue elements (e.g., cells, matrix, vasculature) or aided through images provided by modalities such as histology, CT or MRI. The main input module to the VTS will require this tissue definition along with a user-defined configuration for optical source(s) and detector(s). The computational engine of the VTS will utilize a Monte Carlo (MC) simulation of radiative transport. The VTS output module will provide displays of the spatial distributions of the internal absorption and scattering, light distributions between source(s) and detector(s), and time/spatially-resolved reflectance/transmittance using advanced MC techniques (e.g. perturbation/differential MC, coupled forward-adjoint MC, etc.). Comparison of these results with experimental measurements across spatial scales will validate the models employed. The candidate is an applied mathematician whose expertise lies in Monte Carlo methods for radiative transport. Her career goal is to be capable of leading an independent research program in computational biophotonics and play a critical role in multi-investigator efforts in which computational biophotonics is an integral part. The candidate will be mentored jointly by Profs. Bruce Tromberg and Vasan Venugopalan, who are PI and co-Pi of the Laser Microbeam and Medical Program;a NIH P41 National Biotechnology Resource. The candidate's proposed research activities have strong synergy with the mentors'longstanding research activities in laser-tissue interactions, radiative transport, and optical diagnostics, and microscopy. The proposed research training program will advance her knowledge of cell biology and tissue physiology and provide hands-on experience with optical technologies to characterize tissue on micro- and mesoscopic scales in both laboratory and clinical settings. These training activities will ensure that the VTS is developed in a context where validation is provided via experimental measurements in tissue phantoms and engineered tissues. Relevance: The VTS will provide the Biomedical Optics community a much needed tool to realistically simulate the impact of mesoscopic and microscopic tissue properties and organization on light-tissue interactions. This tool will enable researchers the ability to examine the impact of tissue transformations on optical signals and thereby provide critical guidance for improved design of optical probes/instrumentation used for therapeutic/diagnostic applications.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Mentored Quantitative Research Career Development Award (K25)
Project #
5K25EB007309-05
Application #
8308452
Study Section
Special Emphasis Panel (ZEB1-OSR-E (M1))
Program Officer
Erim, Zeynep
Project Start
2008-08-01
Project End
2013-07-31
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
5
Fiscal Year
2012
Total Cost
$115,002
Indirect Cost
$8,519
Name
University of California Irvine
Department
Engineering (All Types)
Type
Schools of Engineering
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
Gardner, Adam R; Hayakawa, Carole K; Venugopalan, Vasan (2014) Coupled forward-adjoint Monte Carlo simulation of spatial-angular light fields to determine optical sensitivity in turbid media. J Biomed Opt 19:065003
Ranasinghesagara, Janaka C; Hayakawa, Carole K; Davis, Mitchell A et al. (2014) Rapid computation of the amplitude and phase of tightly focused optical fields distorted by scattering particles. J Opt Soc Am A Opt Image Sci Vis 31:1520-30
Hayakawa, Carole K; Spanier, Jerome; Venugopalan, Vasan (2014) Comparative analysis of discrete and continuous absorption weighting estimators used in Monte Carlo simulations of radiative transport in turbid media. J Opt Soc Am A Opt Image Sci Vis 31:301-11
Balu, Mihaela; Kelly, Kristen M; Zachary, Christopher B et al. (2014) Distinguishing between benign and malignant melanocytic nevi by in vivo multiphoton microscopy. Cancer Res 74:2688-97
Balu, Mihaela; Mazhar, Amaan; Hayakawa, Carole K et al. (2013) In vivo multiphoton NADH fluorescence reveals depth-dependent keratinocyte metabolism in human skin. Biophys J 104:258-67
Mittal, Richa; Balu, Mihaela; Krasieva, Tatiana et al. (2013) Evaluation of stimulated Raman scattering microscopy for identifying squamous cell carcinoma in human skin. Lasers Surg Med 45:496-502
Hayakawa, Carole K; Potma, Eric O; Venugopalan, Vasan (2011) Electric field Monte Carlo simulations of focal field distributions produced by tightly focused laser beams in tissues. Biomed Opt Express 2:278-90
Hayakawa, Carole K; Venugopalan, Vasan; Krishnamachari, Vishnu V et al. (2009) Amplitude and phase of tightly focused laser beams in turbid media. Phys Rev Lett 103:043903