This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is: (1) To estimate the oxidative stress in patients with end-stage renal dysfunction (ESRD). We will estimate the oxidative stress (malonyldialdehyde, and carbonyl protein) in the plasma and in skeletal muscle in ESRD patients and matched controls. We will also quantify the antioxidant defenses. (2) To determine the association between oxidative stress, mitochondrial DNA (mtDNA) damage and mitochondrial dysfunction in ESRD patients. We will quantify the mtDNA damage and also mitochondrial function (enzyme activity) and their relation to each other and to oxidative stress. 3) To establish the relationship between abnormal mitochondrial function and apoptosis in the skeletal muscle of patients with ESRD. We will measure the apoptosis by TUNEL assay and also indirectly by activation of caspases.
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