Abstract

The study will assess the effect of amalgam on antibiotic resistance in bacteria. University of Washington will collect and assay oral and urine samples from 150 participants in the University of Washington/Casa Pia Trial (UWCPT) and will collect and analyze quantitative data from these samples.
Specific aims of this study are to determine if there are changes in the prevalence of antibiotic resistant and mercury resistant oral bacteria in children 8-10 years who are treated for dental caries with dental amalgam and those treated with composite filling material between baseline (pre- exposure) to 3 months (immediate post-exposure) to 12 months; to determine if there are differences in changes in the prevalence of antibiotic resistant and mercury resistant oral bacteria for the two groups of participants; to determine if there are changes in the prevalence of antibiotic resistant and mercury resistant enteric flora in the two groups between pre-exposure and the three post-exposure endpoints; to determine if there are any differences in changes in the prevalence of antibiotic resistant and mercury resistant enteric flora for the two groups of participants; and to determine if there is a linkage between the acquisition of mercury resistance and the acquisition of antibiotic resistance genes in oral bacteria and, separately, in enteric bacteria.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research and Development Contracts (N01)
Project #
N01DE072623-006
Application #
6230638
Study Section
Project Start
1997-09-30
Project End
2001-09-29
Budget Start
2000-02-08
Budget End
2001-09-29
Support Year
Fiscal Year
2000
Total Cost
$416,691
Indirect Cost

  Institution

Name
University of Washington
Department
Pathology
Type
Schools of Public Health
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Roberts, M C; Leroux, B G; Sampson, J et al. (2008) Dental amalgam and antibiotic- and/or mercury-resistant bacteria. J Dent Res 87:475-9
Soge, Olusegun O; Beck, Nicola K; White, Teresa M et al. (2008) A novel transposon, Tn6009, composed of a Tn916 element linked with a Staphylococcus aureus mer operon. J Antimicrob Chemother 62:674-80
Ojo, Kayode K; Striplin, Megan J; Ulep, Catherine C et al. (2006) Staphylococcus efflux msr(A) gene characterized in Streptococcus, Enterococcus, Corynebacterium, and Pseudomonas isolates. Antimicrob Agents Chemother 50:1089-91
Ojo, Kayode K; Ruehlen, Nevada L; Close, Natasha S et al. (2006) The presence of a conjugative Gram-positive Tn2009 in Gram-negative commensal bacteria. J Antimicrob Chemother 57:1065-9
Ojo, K K; Ulep, C; Van Kirk, N et al. (2004) The mef(A) gene predominates among seven macrolide resistance genes identified in gram-negative strains representing 13 genera, isolated from healthy Portuguese children. Antimicrob Agents Chemother 48:3451-6
Ojo, Kayode K; Tung, Diane; Luis, Henrique et al. (2004) Gram-positive merA gene in gram-negative oral and urine bacteria. FEMS Microbiol Lett 238:411-6
Luna, Vicki A; Heiken, Marc; Judge, Kathleen et al. (2002) Distribution of mef(A) in gram-positive bacteria from healthy Portuguese children. Antimicrob Agents Chemother 46:2513-7
Starr, J R; White, T C; Leroux, B G et al. (2002) Persistence of oral Candida albicans carriage in healthy Portuguese schoolchildren followed for 3 years. Oral Microbiol Immunol 17:304-10
Luna, V A; Cousin Jr, S; Whittington, W L et al. (2000) Identification of the conjugative mef gene in clinical Acinetobacter junii and Neisseria gonorrhoeae isolates. Antimicrob Agents Chemother 44:2503-6
Luna, V A; Coates, P; Eady, E A et al. (1999) A variety of gram-positive bacteria carry mobile mef genes. J Antimicrob Chemother 44:19-25