Project 3: Mayfield MicroRNA: targets for medication development in alcoholism. Chronic alcohol abuse produces lasting changes in brain function that are manifested as tolerance, physical dependence, craving, and other behavioral changes. The Mayfield Project will test the overall hypothesis that these changes are due to the co-ordinated regulation of alcohol-responsive genes by small non-coding RNAs. This Project will explore the role of these regulatory RNAs using several innovative approaches to alcohol research, including profiling of all known microRNAs (miRNAs), next generation sequencing of unique small RNAs, prediction and validation of miRNAs, mRNA target interactions, expression patterns of miRNAs which may act in combination to regulate mRNA expression and delivery of selected alcohol-related miRNAs to brain.
|Most, D; Ferguson, L; Blednov, Y et al. (2015) The synaptoneurosome transcriptome: a model for profiling the emolecular effects of alcohol. Pharmacogenomics J 15:177-88|
|Cornelison, Garrett L; Mihic, S John (2014) Contaminating levels of zinc found in commonly-used labware and buffers affect glycine receptor currents. Brain Res Bull 100:1-5|
|Mayfield, Jody; Ferguson, Laura; Harris, R Adron (2013) Neuroimmune signaling: a key component of alcohol abuse. Curr Opin Neurobiol 23:513-20|
|Kirson, Dean; Cornelison, Garrett L; Philpo, Ashley E et al. (2013) Physiological concentrations of zinc reduce taurine-activated GlyR responses to drugs of abuse. Neuropharmacology 75:286-94|