The Genetics Core is responsible for providing support for the projects to identify new longevity genes (projects 1 &2), to delineate genotypes of interest in the individual longitudinal projects (Projects 3 &4), and to validate the genetic data in an independent non-Jewish population and our new recruits (Projects 3&4). This Core will isolate, purify, send for assay or store genomic DNA obtained from peripheral blood samples from all subjects collected as part of this program. Whole genome SNP analysis will be performed on the purified DMAs of 250 centenarians and 250 unrelated younger control, using the Affimetrix 500K mapping set (Project 1). As part of Project 1 we will determine the frequency of significant SNPs in 750 longevity genes and 750 buffered-killing'genes, from the genomic DNA in the offspring of centenarians and more centenarians and control by partnering with Illumina Corporation. For this study, we will grid the subjects DNA samples of and send to Illumina Services for genotyping a total of 1536 custom SNPs in their GoldenGate array. Among the SNPs are these find significant in Project 1 and 2, and for CETP, APOC3, ADIPOQ and 2 APOE for subjects in projects 3 and 4. Pyrosequencing will be used to perform high throughout screening of DNAs from large numbers of study subjects with limited numbers of SNPs in the GH/IGF pathway identified in Projects 2 (years 3-5). The genetic information obtained by this Core will be communicated to the Statistical and Bioinformatics Core, where it will become available to all investigators. Dr. Morrow is currently the Faculty Advisor of the DNA Processing Core, which has been functioning to store cell lines and isolate DNA from blood samples for the last ten years. Similarly, she also oversees the DNA Sequencing and Genotyping Core, in which Mr. David Reynolds is the Director. Both of these Cores are housed in the Department of Molecular Genetics at AECOM. Thus, the Genetics Core proposed here, in effect, combines two well-established functioning core services at our institution with extensive experience in technologies required for performing the studies outlined in this section and in achieving the goals of this application.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
3P01AG027734-05S3
Application #
8544712
Study Section
Special Emphasis Panel (ZAG1-ZIJ-2)
Project Start
Project End
Budget Start
2011-08-01
Budget End
2012-07-31
Support Year
5
Fiscal Year
2012
Total Cost
$19,045
Indirect Cost
$7,641
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Ezzati, Ali; Wang, Cuiling; Lipton, Richard B et al. (2017) Association Between Vascular Pathology and Rate of Cognitive Decline Independent of Alzheimer's Disease Pathology. J Am Geriatr Soc 65:1836-1841
Manning, Alisa (see original citation for additional authors) (2017) A Low-Frequency Inactivating AKT2 Variant Enriched in the Finnish Population Is Associated With Fasting Insulin Levels and Type 2 Diabetes Risk. Diabetes 66:2019-2032
Sundermann, Erin E; Katz, Mindy J; Lipton, Richard B (2017) Sex Differences in the Relationship between Depressive Symptoms and Risk of Amnestic Mild Cognitive Impairment. Am J Geriatr Psychiatry 25:13-22
Seng, Elizabeth K; Buse, Dawn C; Klepper, Jaclyn E et al. (2017) Psychological Factors Associated With Chronic Migraine and Severe Migraine-Related Disability: An Observational Study in a Tertiary Headache Center. Headache 57:593-604
Kato, Kaori; Zweig, Richard; Schechter, Clyde B et al. (2016) Positive attitude toward life, emotional expression, self-rated health, and depressive symptoms among centenarians and near-centenarians. Aging Ment Health 20:930-9
Roshandel, Delnaz; Klein, Ronald; Klein, Barbara E K et al. (2016) New Locus for Skin Intrinsic Fluorescence in Type 1 Diabetes Also Associated With Blood and Skin Glycated Proteins. Diabetes 65:2060-71
Sundermann, Erin E; Biegon, Anat; Rubin, Leah H et al. (2016) Better verbal memory in women than men in MCI despite similar levels of hippocampal atrophy. Neurology 86:1368-76
Perice, Leland; Barzilai, Nir; Verghese, Joe et al. (2016) Lower circulating insulin-like growth factor-I is associated with better cognition in females with exceptional longevity without compromise to muscle mass and function. Aging (Albany NY) 8:2414-2424
Sundermann, Erin Elizabeth; Wang, Cuiling; Katz, Mindy et al. (2016) Cholesteryl ester transfer protein genotype modifies the effect of apolipoprotein ?4 on memory decline in older adults. Neurobiol Aging 41:200.e7-200.e12
Baskovich, Brett; Hiraki, Susan; Upadhyay, Kinnari et al. (2016) Expanded genetic screening panel for the Ashkenazi Jewish population. Genet Med 18:522-8

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