The purpose of the Mass Spectrometry Core is to provide support for the projects by quantitativedetermination of steroids by liquid chromatography - tandem mass spectrometry (LC/MS/MS) as its majoractivity. Additionally, specialized services will include identification of proteins separated by gelelectrophoresis, identification and quantification of Ca2+-channel/receptor isotypes and their oxidationsensitivecysteine (Cys) thiols critical to function by methods based on electrospray ionization (ESI) andLC/MS/MS. These support needs were identified by the project leaders, and the requirements/methods werediscussed in details through interactions with the core director to select the most appropriate ones to beimplemented as core services. Equipments made available on a shared basis are a quadrupole ion-trapmass spectrometer for steroid analysis and a linear ion-trap - Fourier-transform ion-cyclotron resonanceinstrument for proteomics-related work. The systems also incorporate advanced liquid chrom atographs forsample separations. Core B will provide expert leadership, specialists and technical staff to perform the coreservices and provide full reports on the results of quantitative steroid analyses and on protein identification,quantification to the project leaders and research personnel.
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