Core B - David C. Schultz, Ph. D. The Protein and Vector Production Core will be responsible for time efficient and expert technical assistance in the management of project resources, protein expression in bacteria and baculovirus infected insect cells, purification of recombinant proteins to homogeneity, and production of high-titer stocks of retroviruses (e.g. lentiviruses) for delivery of shRNA and cDNAs to mammalian cells. Expertise in all aspects of vector technology for protein expression, baculovirus generation, recombinant protein expression, and affinity and conventional chromatography approaches to protein purification, and production of infectious lenti- /retroviruses will be provided to all projects on a continual basis for the lifetime of grant support. The Core personnel are highly trained technical experts in all areas of the proposed support services to be provided to the individual projects. The centralization and standardization of these practices under the direction of very experienced staff allows for high-throughput, large-volume, protein expression services, including quality assurance and control procedures to ensure efficient, consistent production and purification of high quality recombinant proteins. Furthermore, confinement of retrovirus (e.g. lentivirus) production to a centralized biosafety level 2 (BSL2) unit provides increased biosafety, prevention of aerosolized viruses from contaminating incubators and parental cultures of cell lines, and quality control in the production of virus stocks to be used in experiments. In addition, the collection of projects in this proposal is expected to generate unique research resources that will be required for programmatic interactions. The Core will function as a repository for collection, initial processing, storage, maintenance, and distribution of newly developed materials. In this manner, resources can be reliably tracked to ensure quality control, adherence to guidelines for resource sharing, and consistency in usage within programmatic interactions and the greater scientific community.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG031862-04
Application #
8235003
Study Section
Special Emphasis Panel (ZAG1)
Project Start
Project End
Budget Start
2011-03-01
Budget End
2012-02-29
Support Year
4
Fiscal Year
2011
Total Cost
$130,083
Indirect Cost
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Berson, Amit; Sartoris, Ashley; Nativio, Raffaella et al. (2017) TDP-43 Promotes Neurodegeneration by Impairing Chromatin Remodeling. Curr Biol 27:3579-3590.e6
Bonini, Nancy M; Berger, Shelley L (2017) The Sustained Impact of Model Organisms-in Genetics and Epigenetics. Genetics 205:1-4
Mews, Philipp; Donahue, Greg; Drake, Adam M et al. (2017) Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory. Nature 546:381-386
Ricketts, M Daniel; Marmorstein, Ronen (2017) A Molecular Prospective for HIRA Complex Assembly and H3.3-Specific Histone Chaperone Function. J Mol Biol 429:1924-1933
Simithy, Johayra; Sidoli, Simone; Yuan, Zuo-Fei et al. (2017) Characterization of histone acylations links chromatin modifications with metabolism. Nat Commun 8:1141
Yang, Ting-Lin B; Chen, Qijun; Deng, Jennifer T et al. (2017) Mutual reinforcement between telomere capping and canonical Wnt signalling in the intestinal stem cell niche. Nat Commun 8:14766
Feng, Zijie; Wang, Lei; Sun, Yanmei et al. (2017) Menin and Daxx Interact to Suppress Neuroendocrine Tumors through Epigenetic Control of the Membrane Metallo-Endopeptidase. Cancer Res 77:401-411
Vizioli, Maria Grazia; Adams, Peter D (2016) Senescence Can Be BETter without the SASP? Cancer Discov 6:576-8
Sen, Payel; Shah, Parisha P; Nativio, Raffaella et al. (2016) Epigenetic Mechanisms of Longevity and Aging. Cell 166:822-839
Pchelintsev, Nikolay A; Adams, Peter D; Nelson, David M (2016) Critical Parameters for Efficient Sonication and Improved Chromatin Immunoprecipitation of High Molecular Weight Proteins. PLoS One 11:e0148023

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