instmctions); The Animal, Cell and Tissue Culture Core (Core B) will be responsible for providing investigators of the Program Project (PPG) with animal models, tissues and cells necessary to achieve their specific aims. Experiments of this PPG focus on identifying mechanisms responsible for differences in longevity between the species of rodents. Rodents offer a unique opportunity for such comparative study of longevity because of over 10-fold differences in life spans represented within a single mammalian order. For example, short- lived rodents, such as mouse and rat, live 3-5 years, while long-lived rodents, such as naked mole rat, blind mole rat, grey squirrel, beaver, and porcupine have maximum lifespans ranging from 21 to 32 years. To enable the study of molecular mechanisms of longevity in these animals, it is essential to provide access to biological materials from several individuals of multiple rodent species. Core B will maintain and replenish, as needed, the collection of tissues and primary cells from 18 species of rodents. The Core will provide project investigators with tissues, cells, DNA, and RNA, and perform mutagenic treatments of cells. In addition. Core B will maintain mice and naked mole rats for the use by project investigators. Considering the effort needed to collect and establish cell cultures from non-standard organisms that are not commercially available, the centralized resource will save time and effort, and reduce costs! Maintaining the centralized rodent collection will also standardize culture conditions, quality control and biological samples for use across PPG projects, improve reproducibility of results and allow the analysis of the same individual animals by several assays and projects, as well as minimize animal use.
; The Animal, Cell and Tissue Culture Core (Core B) will maintain animal, cell and tissue collection for the project investigators. This resource is essential for all the four projects to achieve their specific aims.
|Ma, Siming; Upneja, Akhil; Galecki, Andrzej et al. (2016) Cell culture-based profiling across mammals reveals DNA repair and metabolism as determinants of species longevity. Elife 5:|
|Quispe-Tintaya, Wilber; Gorbacheva, Tatyana; Lee, Moonsook et al. (2016) Quantitative detection of low-abundance somatic structural variants in normal cells by high-throughput sequencing. Nat Methods 13:584-6|
|Dokukin, Maxim; Ablaeva, Yulija; Kalaparthi, Vivekanand et al. (2016) Pericellular Brush and Mechanics of Guinea Pig Fibroblast Cells Studied with AFM. Biophys J 111:236-46|
|Gorbunova, Vera; Seluanov, Andrei (2016) DNA double strand break repair, aging and the chromatin connection. Mutat Res 788:2-6|
|Patrick, Alison; Seluanov, Michael; Hwang, Chaewon et al. (2016) Sensitivity of primary fibroblasts in culture to atmospheric oxygen does not correlate with species lifespan. Aging (Albany NY) 8:841-7|
|White, Ryan R; Vijg, Jan (2016) Do DNA Double-Strand Breaks Drive Aging? Mol Cell 63:729-38|
|Tian, Xiao; Azpurua, Jorge; Ke, Zhonghe et al. (2015) INK4 locus of the tumor-resistant rodent, the naked mole rat, expresses a functional p15/p16 hybrid isoform. Proc Natl Acad Sci U S A 112:1053-8|
|Ma, Siming; Yim, Sun Hee; Lee, Sang-Goo et al. (2015) Organization of the Mammalian Metabolome according to Organ Function, Lineage Specialization, and Longevity. Cell Metab 22:332-43|
|Ma, Siming; Lee, Sang-Goo; Kim, Eun Bae et al. (2015) Organization of the Mammalian Ionome According to Organ Origin, Lineage Specialization, and Longevity. Cell Rep 13:1319-26|
|MacRae, Sheila L; Zhang, Quanwei; Lemetre, Christophe et al. (2015) Comparative analysis of genome maintenance genes in naked mole rat, mouse, and human. Aging Cell 14:288-91|
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