Laboratory Core B's primary aim is to provide accurate, timely, centralized diagnostic test services to the projects and to Clinical Core A using the facilities of the Virology Division at University of Washington's ("UW") Department of Laboratory Medicine. The UW Virology Lab at Seattle Children's'Hospital (UW-SCH) will provide HSV serology, HSV culture, arid banking of sera and viral isolates. UW-SCH will also direct the serology testing for other viral pathogens that is required by Projects 1, 2, and 4. Dr. Rhoda Ashley Morrow, Core B Leader, directed the UW-SCH lab from 1983-2010 and continues to direct its Serology Section. The second Core B lab, "UW-PCR" is located within the UW Virology Division's Molecular Diagnostic Laboratory. This lab detects viral pathogens by amplifying their genetic material by polymerase chain reaction (PCR) methods. Dr. MeeiLi Huang has developed several generations of HSV PCR tests since the mid-1990s and will continue to provide the leadership for this testing. UW-PCR staff will coordinate the sample preparation for HLA markers (Projects 2, 3 and 4) and will manage specimen repositories of HSV DNA and human DNA in support of the proposed projects.
The second aim of the Laboratory Core is to direct training and proficiency testing for Project 1's laboratory functions in Nairobi for Project 1 and to oversee quality control development for testing described in individual projects (e.g.;computerized target cell counting for Projects 1,3 4 and cytokine immune factors for Project 1). Dr. Morrow is experienced in training staff and developing proficiency testing programs in laboratories both locally and outside the United States, including in African sites. The third and final aim is to develop and standardize novel tests for Project 1 for antibody function and for the detection of semen in samples from the genital tract of female subjects. The Core ensures that the Scientific Projects can obtain results from high throughput laboratory testing without redundant effort and waste of resources.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
2P01AI030731-23
Application #
8565781
Study Section
Special Emphasis Panel (ZAI1-LR-M (M1))
Project Start
Project End
Budget Start
2013-07-05
Budget End
2014-06-30
Support Year
23
Fiscal Year
2013
Total Cost
$289,993
Indirect Cost
$88,047
Name
University of Washington
Department
Type
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
Magaret, Amalia S; Mujugira, Andrew; Hughes, James P et al. (2016) Effect of Condom Use on Per-act HSV-2 Transmission Risk in HIV-1, HSV-2-discordant Couples. Clin Infect Dis 62:456-61
Oseso, Linda; Magaret, Amalia S; Jerome, Keith R et al. (2016) Attitudes and Willingness to Assume Risk of Experimental Therapy to Eradicate Genital Herpes Simplex Virus Infection. Sex Transm Dis 43:566-71
Magaret, A; Dong, L; John, M et al. (2016) HLA Class I and II alleles, heterozygosity and HLA-KIR interactions are associated with rates of genital HSV shedding and lesions. Genes Immun 17:412-418
Jing, Lichen; Laing, Kerry J; Dong, Lichun et al. (2016) Extensive CD4 and CD8 T Cell Cross-Reactivity between Alphaherpesviruses. J Immunol 196:2205-18
Manguro, Griffins O; Masese, Linnet N; Deya, Ruth W et al. (2016) Genital HSV Shedding among Kenyan Women Initiating Antiretroviral Therapy. PLoS One 11:e0163541
Ramchandani, Meena; Kong, Marlene; Tronstein, Elizabeth et al. (2016) Herpes Simplex Virus Type 1 Shedding in Tears and Nasal and Oral Mucosa of Healthy Adults. Sex Transm Dis 43:756-760
Milman, Neta; Zhu, Jia; Johnston, Christine et al. (2016) In Situ Detection of Regulatory T Cells in Human Genital Herpes Simplex Virus Type 2 (HSV-2) Reactivation and Their Influence on Spontaneous HSV-2 Reactivation. J Infect Dis 214:23-31
Tjernlund, Annelie; Burgener, Adam; Lindvall, Jessica M et al. (2016) In Situ Staining and Laser Capture Microdissection of Lymph Node Residing SIV Gag-Specific CD8+ T cells--A Tool to Interrogate a Functional Immune Response Ex Vivo. PLoS One 11:e0149907
Nason, Martha C; Patel, Eshan U; Kirkpatrick, Allison R et al. (2016) Immunological Signaling During Herpes Simplex Virus-2 and Cytomegalovirus Vaginal Shedding After Initiation of Antiretroviral Treatment. Open Forum Infect Dis 3:ofw073
Bender Ignacio, Rachel A; Goldman, Jason D; Magaret, Amalia S et al. (2016) Patterns of human herpesvirus-8 oral shedding among diverse cohorts of human herpesvirus-8 seropositive persons. Infect Agent Cancer 11:7

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