Abstract

The vector core provides clinical investigators with services relating to generation, manufacturing and validation of clinical grade vectors and also validation of transduced cells for clinical trials. The core provides the vectors being used for modification of human autologous cytotoxic T cells for the clinical trials described in project 1 and the vectors containing HIV intracellular vaccines constructs for the clinical trial in project 2. All vectors are produced in accordance with good laboratory practices (GLP) and good manufacturing practices (GMP) consistent with FDA requirements. In addition to manufacturing clinical grade vectors, the vector core is responsible for regulatory filings with the FDA relating to manufacturing and clinical use of these vectors. The vector core also assists investigators with obtaining regulatory approval for the clinical trials described in Projects 1 and 2.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI043650-03
Application #
6344657
Study Section
Project Start
2000-09-01
Project End
2001-08-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
3
Fiscal Year
2000
Total Cost
$251,290
Indirect Cost

  Institution

Name
Fred Hutchinson Cancer Research Center
Department
Type
DUNS #
075524595
City
Seattle
State
WA
Country
United States
Zip Code
98109

  Publications

Pollack, Seth M; Jones, Robin L; Farrar, Erik A et al. (2014) Tetramer guided, cell sorter assisted production of clinical grade autologous NY-ESO-1 specific CD8(+) T cells. J Immunother Cancer 2:36
Ochsenbein, Adrian F; Riddell, Stanley R; Brown, Michele et al. (2004) CD27 expression promotes long-term survival of functional effector-memory CD8+ cytotoxic T lymphocytes in HIV-infected patients. J Exp Med 200:1407-17
Cooper, Laurence J N; Topp, Max S; Pinzon, Cris et al. (2004) Enhanced transgene expression in quiescent and activated human CD8+ T cells. Hum Gene Ther 15:648-58
Topp, Max S; Riddell, Stanley R; Akatsuka, Yoshiki et al. (2003) Restoration of CD28 expression in CD28- CD8+ memory effector T cells reconstitutes antigen-induced IL-2 production. J Exp Med 198:947-55
Cheng, Laurence E; Ohlen, Claes; Nelson, Brad H et al. (2002) Enhanced signaling through the IL-2 receptor in CD8+ T cells regulated by antigen recognition results in preferential proliferation and expansion of responding CD8+ T cells rather than promotion of cell death. Proc Natl Acad Sci U S A 99:3001-6
Lewinsohn, Deborah A; Lines, Rebecca; Lewinsohn, David M et al. (2002) HIV-1 Vpr does not inhibit CTL-mediated apoptosis of HIV-1 infected cells. Virology 294:13-21
Cheng, Laurence E; Greenberg, Philip D (2002) Selective delivery of augmented IL-2 receptor signals to responding CD8+ T cells increases the size of the acute antiviral response and of the resulting memory T cell pool. J Immunol 169:4990-7
Truong, Hong-Ha M; Berrey, M Michelle; Shea, Theresa et al. (2002) Concordance between HIV source partner identification and molecular confirmation in acute retroviral syndrome. J Acquir Immune Defic Syndr 29:232-43
Bonini, C; Lee, S P; Riddell, S R et al. (2001) Targeting antigen in mature dendritic cells for simultaneous stimulation of CD4+ and CD8+ T cells. J Immunol 166:5250-7
Georges, G E; Storb, R; Bruno, B et al. (2001) Engraftment of DLA-haploidentical marrow with ex vivo expanded, retrovirally transduced cytotoxic T lymphocytes. Blood 98:3447-55

Showing the most recent 10 out of 13 publications