The Flow Cytometry Core's goal is to optimize Program Project scientific productivity by providing access to state-of-the-art equipment, novel reagents, and specialized expertise in all aspects of flow cytometry. Both Projects and the Tetramer Core rely heavily on flow cytometry techniques. Program Project support allows the Flow Core to contract with the UMMS Flow Cytometry Facility to provide Project Investigators with preferential access to unique services (including cell sorting and complex flow cytometry analytical experiments) and specialized facilities that are not available in individual Investigators'laboratories at reduced rates. The Flow Cytometry Core also works actively with Program Project investigators to develop state-of-the-art protocols and experimental approaches and works with the Tetramer Core in the generation and validation of new reagents. Finally, the Flow Core provides training and workshops to Project and Core investigators and our trainees (post-doctoral fellows and graduate students).
A key goal of this Program Project is to use the EBV model of persistent infection to characterize the development and evolution of virus-specific and cross-reactive CD8+ T cells. These studies will improve our understanding of the development of Cd8+ T cell responses and will allow us to better understand how EBV- specific and cross-reactive CD8+ T cells contribute to either control of viral replication or immune pathology. The Flow Cytometry Core provides access to state-of-the-art equipment, novel reagents, and specialized flow cytometry expertise to all Project Investigators and the Tetramer Core.
|Aslan, Nuray; Watkin, Levi B; Gil, Anna et al. (2017) Severity of Acute Infectious Mononucleosis Correlates with Cross-Reactive Influenza CD8 T-Cell Receptor Repertoires. MBio 8:|
|Song, InYoung; Gil, Anna; Mishra, Rabinarayan et al. (2017) Broad TCR repertoire and diverse structural solutions for recognition of an immunodominant CD8+ T cell epitope. Nat Struct Mol Biol 24:395-406|
|Watkin, Levi B; Mishra, Rabinarayan; Gil, Anna et al. (2017) Unique influenza A cross-reactive memory CD8 T-cell receptor repertoire has a potential to protect against EBV seroconversion. J Allergy Clin Immunol 140:1206-1210|
|Gil, Anna; Yassai, Maryam B; Naumov, Yuri N et al. (2015) Narrowing of human influenza A virus-specific T cell receptor ? and ? repertoires with increasing age. J Virol 89:4102-16|
|Gil, Anna; Kenney, Laurie L; Mishra, Rabinarayan et al. (2015) Vaccination and heterologous immunity: educating the immune system. Trans R Soc Trop Med Hyg 109:62-9|
|Greenough, Thomas C; Straubhaar, Juerg R; Kamga, Larisa et al. (2015) A Gene Expression Signature That Correlates with CD8+ T Cell Expansion in Acute EBV Infection. J Immunol 195:4185-97|
|Renzette, Nicholas; Somasundaran, Mohan; Brewster, Frank et al. (2014) Epstein-Barr virus latent membrane protein 1 genetic variability in peripheral blood B cells and oropharyngeal fluids. J Virol 88:3744-55|
|Chen, Alex T; Cornberg, Markus; Gras, Stephanie et al. (2012) Loss of anti-viral immunity by infection with a virus encoding a cross-reactive pathogenic epitope. PLoS Pathog 8:e1002633|
|Tracy, Sean I; Kakalacheva, Kristina; Lunemann, Jan D et al. (2012) Persistence of Epstein-Barr virus in self-reactive memory B cells. J Virol 86:12330-40|
|O'Bryan, Joel M; Potts, James A; Bonkovsky, Herbert L et al. (2011) Extended interferon-alpha therapy accelerates telomere length loss in human peripheral blood T lymphocytes. PLoS One 6:e20922|
Showing the most recent 10 out of 36 publications