Abstract

All three projects of the Program Project will continuously use recombinant murine and human CD1d molecules for lipid binding studies, crystallography, biochemistry and for the making of tetramers to stain specifically NK1.1 T cells. This production will be best ensured by a Core Facility that can express, purify, biotinylate and distribute the reagent. The Core will also be in charge of quality control by doing for each batch a series of tests that will include: fluorescent binding assay with PE-FITC, control of biotinylation by immuno-depletion on streptavidin-agarose beads, and tetramerization/FACS staining of the NK1.1 + DN32.D3 cell line. The standardization of protocols will guarantee reproducibility between batches. The Core will also monitor storage of recombinant CD1d and usage by the three different groups. No priority will be given to any one them, unless limitations in production impose us to. Finally, the existence of this Core will reduce the cost of production dramatically.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI053725-10
Application #
8376760
Study Section
Special Emphasis Panel (ZAI1-CL-I)
Project Start
Project End
2014-03-31
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
10
Fiscal Year
2012
Total Cost
$81,466
Indirect Cost
$2,444

  Institution

Name
University of Chicago
Department
Type
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637

  Publications

Deng, S; Bai, L; Reboulet, R et al. (2014) A peptide-free, liposome-based oligosaccharide vaccine, adjuvanted with a natural killer T cell antigen, generates robust antibody responses in vivo. Chem Sci 5:1437-1441
Kain, Lisa; Webb, Bill; Anderson, Brian L et al. (2014) The identification of the endogenous ligands of natural killer T cells reveals the presence of mammalian ?-linked glycosylceramides. Immunity 41:543-54
Tefit, Josianne Nitcheu; Crabé, Sandrine; Orlandini, Bernard et al. (2014) Efficacy of ABX196, a new NKT agonist, in prophylactic human vaccination. Vaccine 32:6138-45
Bai, Li; Deng, Shenglou; Reboulet, Rachel et al. (2013) Natural killer T (NKT)-B-cell interactions promote prolonged antibody responses and long-term memory to pneumococcal capsular polysaccharides. Proc Natl Acad Sci U S A 110:16097-102
Anderson, Brian L; Teyton, Luc; Bendelac, Albert et al. (2013) Stimulation of natural killer T cells by glycolipids. Molecules 18:15662-88
Luoma, Adrienne M; Castro, Caitlin D; Mayassi, Toufic et al. (2013) Crystal structure of V?1 T cell receptor in complex with CD1d-sulfatide shows MHC-like recognition of a self-lipid by human ?? T cells. Immunity 39:1032-42
Constantinides, Michael G; Bendelac, Albert (2013) Transcriptional regulation of the NKT cell lineage. Curr Opin Immunol 25:161-7
Freigang, Stefan; Kain, Lisa; Teyton, Luc (2013) Transport and uptake of immunogenic lipids. Mol Immunol 55:179-81
Bai, Li; Picard, Damien; Anderson, Brian et al. (2012) The majority of CD1d-sulfatide-specific T cells in human blood use a semiinvariant V?1 TCR. Eur J Immunol 42:2505-10
Bai, Li; Constantinides, Michael G; Thomas, Seddon Y et al. (2012) Distinct APCs explain the cytokine bias of ?-galactosylceramide variants in vivo. J Immunol 188:3053-61

Showing the most recent 10 out of 33 publications