The Specimen Acquisition and Distribution Core will be responsible for obtaining, tracking and allocating blood and tissue samples (colon, ileum, lymph node, plasma and PBMC) for use by the individual projects. The function is critical to the overall development of this PO1 as the Core's support will allow investigators for each of the projects to focus on the generation of preliminary data derived from specimens provided by the Core. The Core will coordinate tissue procurement at Rush University Medical Center (RUMC), University of California-San Francisco (UCSF), and Case Western Reserve University (CWRU), and will oversee distribution of tissue and body fluid samples to Projects 1, 2, and 4. These objectives will be accomplished by implementing an established internet-based communication system to provide real time display of available specimens and an agile online requisition system. All of the routine collection methods are in place at each of the sites of this Core. The ongoing data collection systems established at the sites allow for ready identification of eligible patients who can be scheduled for biopsies within a few weeks. The infrastructure that is in place at UCSF will allow for the efficient and timely identification of unique and rare patients (e.g. "elite controllers"). Similar systems are in place at the RUMC and CWRU sites for obtaining lymph node and gut tissue samples. In addition to the primary function of the Core in obtaining tissue, the Core will ensure the availability of cryopreserved cells and plasma specimen obtained at each site, and will process and ship the urine specimens needed for the intestinal permeability studies of Project 2. The Core will also obtain relevant clinical information on subjects including current and previous antiretroviral therapy, CD4+ T-cell counts, HIV-1 RNA levels and co-morbid conditions. The overall specific aims for the Core are: 1. Coordinate the timely and efficient acquisition, preparation and transportation of high-quality tissue specimens from the clinical sites at RUMC, UCSF and CWRU to the appropriate project principal investigators. 2. Assure that uniform clinical information is obtained at all clinical sites and that standardized data collection tools are used. 3. Maintain a defined set of standardized data on tissue and cell types, and volumes available for distribution in the repository at all times. 4. Track the collective inventory of viably cryopreserved cells and blood specimens at each participating site for use within the Program project.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI076174-05
Application #
8377465
Study Section
Special Emphasis Panel (ZAI1-PRJ-A)
Project Start
Project End
2014-07-31
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
5
Fiscal Year
2012
Total Cost
$333,247
Indirect Cost
$41,493
Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106
Luo, Zhenwu; Ma, Lei; Zhang, Lumin et al. (2016) Key differences in B cell activation patterns and immune correlates among treated HIV-infected patients versus healthy controls following influenza vaccination. Vaccine 34:1945-55
Shive, Carey L; Clagett, Brian; McCausland, Marie R et al. (2016) Inflammation Perturbs the IL-7 Axis, Promoting Senescence and Exhaustion that Broadly Characterize Immune Failure in Treated HIV Infection. J Acquir Immune Defic Syndr 71:483-92
Panigrahi, Soumya; Freeman, Michael L; Funderburg, Nicholas T et al. (2016) SIV/SHIV Infection Triggers Vascular Inflammation, Diminished Expression of Krüppel-like Factor 2 and Endothelial Dysfunction. J Infect Dis 213:1419-27
McGinty, Tara; Mirmonsef, Paria; Mallon, Patrick W G et al. (2016) Does systemic inflammation and immune activation contribute to fracture risk in HIV? Curr Opin HIV AIDS 11:253-60
Siedner, Mark J; Kim, June-Ho; Nakku, Ruth Sentongo et al. (2016) HIV infection and arterial stiffness among older-adults taking antiretroviral therapy in rural Uganda. AIDS 30:667-70
Pandiyan, Pushpa; Younes, Souheil-Antoine; Ribeiro, Susan Pereira et al. (2016) Mucosal Regulatory T Cells and T Helper 17 Cells in HIV-Associated Immune Activation. Front Immunol 7:228
Siedner, Mark J; Kim, June-Ho; Nakku, Ruth Sentongo et al. (2016) Persistent Immune Activation and Carotid Atherosclerosis in HIV-Infected Ugandans Receiving Antiretroviral Therapy. J Infect Dis 213:370-8
Fu, P; Hughes, J; Zeng, G et al. (2016) A comparative investigation of methods for longitudinal data with limits of detection through a case study. Stat Methods Med Res 25:153-66
Mudd, Joseph C; Panigrahi, Soumya; Kyi, Benjamin et al. (2016) Inflammatory Function of CX3CR1+ CD8+ T Cells in Treated HIV Infection Is Modulated by Platelet Interactions. J Infect Dis 214:1808-1816
Lee, Sulggi A; Mefford, Joel A; Huang, Yong et al. (2016) Host genetic predictors of the kynurenine pathway of tryptophan catabolism among treated HIV-infected Ugandans. AIDS 30:1807-15

Showing the most recent 10 out of 87 publications