Autoimmune patients undergoing B cell depletion therapy (BCDT) with Rituximab can enter clinical remission for years, while others experience only transient relief (months). Although we do not understand why some patients respond well to BCDT and others do not, it is clear that B cells are pathogenic in a subset of patients. Clinical data show that BCDT can be efficacious without significantly reducing autoantibody titer, suggesting that B cells cause pathology via by another mechanism. Therefore, it is important to identify the Ab-independent functions of B cells and understand how B cells performing these functions contribute to pathology in autoimmune disease. However, these efforts have been hampered because we understand so little about the Ab-independent effector functions of B cells. Our data show that one potentially important effector function of B cells is to secrete cytokines. Indeed, we know that cytokines made specifically by B lineage cells regulate humoral and cellular immune responses in vivo. Based on these data, we hypothesize that cytokine-producing B cell effectors (Beff) likely contribute to immune responses to pathogens as well as autoantigens and that Beff cells can be either protective or damaging depending on the immune microenvironment and the cytokine secreted by the Beff cells. However, without until recently, it has been difficult to experimentally address this hypothesis, as we were not able to track cytokine-producing Beff cells in vivo. Importantly, we have now identified a novel subset of IFNy-producing Beff cells that develop in response to influenza infection and are inappropriately expanded in a mouse model of SLE (YaaFcyRllb-/- or Yaa.R2 mice). These data now provide us with the unique opportunity to address our original hypothesis. Therefore goals of this proposal are to (i) determine the factors that regulate the development of IFNy Beff cells in infectious and autoimmune settings and (ii) identify the functional role(s) of these cells in immune responses directed against pathogens and autoantigens.
Approximately 3-5% of Americans suffer from autoimmune disease and effective treatments for many patients are lacking. One promising therapy for autoimmune disease is Rituximab, an antibody that depletes B cells from pro-B cells to plasmablasts. The goals of this proposal are to understand how B cells mediate pathology in autoimmune disease.
|Meednu, Nida; Zhang, Hengwei; Owen, Teresa et al. (2016) Production of RANKL by Memory B Cells: A Link Between B Cells and Bone Erosion in Rheumatoid Arthritis. Arthritis Rheumatol 68:805-16|
|Rahimi, Homaira; Bell, Richard; Bouta, Echoe M et al. (2016) Lymphatic imaging to assess rheumatoid flare: mechanistic insights and biomarker potential. Arthritis Res Ther 18:194|
|Kuzin, Igor I; Kates, Stephen L; Ju, Yawen et al. (2016) Increased numbers of CD23(+) CD21(hi) Bin-like B cells in human reactive and rheumatoid arthritis lymph nodes. Eur J Immunol 46:1752-7|
|Newell, K A; Asare, A; Sanz, I et al. (2015) Longitudinal studies of a B cell-derived signature of tolerance in renal transplant recipients. Am J Transplant 15:2908-20|
|Adlowitz, Diana G; Barnard, Jennifer; Biear, Jamie N et al. (2015) Expansion of Activated Peripheral Blood Memory B Cells in Rheumatoid Arthritis, Impact of B Cell Depletion Therapy, and Biomarkers of Response. PLoS One 10:e0128269|
|Bird, Anna K; Meednu, Nida; Anolik, Jennifer H (2015) New insights into B cell biology in systemic lupus erythematosus and SjÃ¶gren's syndrome. Curr Opin Rheumatol 27:461-7|
|Wilmore, Joel R; Asito, Amolo S; Wei, Chungwen et al. (2015) AID expression in peripheral blood of children living in a malaria holoendemic region is associated with changes in B cell subsets and Epstein-Barr virus. Int J Cancer 136:1371-80|
|Halliley, Jessica L; Tipton, Christopher M; Liesveld, Jane et al. (2015) Long-Lived Plasma Cells Are Contained within the CD19(-)CD38(hi)CD138(+) Subset in Human Bone Marrow. Immunity 43:132-45|
|Bouta, Echoe M; Li, Jie; Ju, Yawen et al. (2015) The role of the lymphatic system in inflammatory-erosive arthritis. Semin Cell Dev Biol 38:90-7|
|Wei, Chungwen; Jenks, Scott; Sanz, IÃ±aki (2015) Polychromatic flow cytometry in evaluating rheumatic disease patients. Arthritis Res Ther 17:46|
Showing the most recent 10 out of 53 publications