The purpose of the Immuno-Epidemiology Research Laboratory Core (Core C) is to provide a technical, intellectual and physical resource for the investigators in this Program Project Grant application. This laboratory has substantial experience performing all of the tasks and assays that are proposed for this Core in the present proposal and has been providing these services for the WHEALS project since its inception. As the central processing laboratory, Projects 1, 2, 3 and 4 will rely on Core C to process, track, store and/or ship archived and newly acquired blood, stool and dust specimens. Additionally, for Project 2, Core C will perform the isolation, culture and stimulation of peripheral blood mononuclear cells (PBMCs);dendritic cell and regulatory T cell phenotyping by multicolor flow cytometry;RNA analysis by realfime PCR;and Bio-Plex assay of secreted cytokines following stimulation of cultured PBMCs.
The Specific Aims of Core C are: 1) To serve as the central collection, storage and processing laboratory for blood and dust samples collected in Projects 1, 2, 3 and 4;to ship plasma and dust samples to the dust and IgE Analysis Core (Core E) for Projects 2, 3, and 4;to ship stool samples to the Microbial Community Analysis Core (Core D) for Projects 1, 2 and 4;and, to ship processed dust samples collected in Project 2 to Dr. Lukacs'lab for use in Project 3, 2) To perform validated flow cytometric assays for phenotyping of dendritic cells and Tregs, and to perform validated Bio-Plex assays of secreted cytokines in stimulated PBMC culture supernatants for Project 2, in an efficient, reliable and consistent fashion, 3) To sen/e as a biorepository for plasma, DNA, RNA, dust, stool and other biological samples for use in future studies, 4) To provide data obtained from flow cytometry and Bio-Plex analyses to the Biostatistics and Data Management Core (Core B) for integration with data from Cores D and E and other data sources for statistical analyses for manuscripts and additional grant applications, and 5) To collaborate with the other investigators in the interpretation and integration of immunologic assay results into publications.

Public Health Relevance

A centralized laboratory Core will increase efficiency, consistency, and reliability and decrease overall costs by providing specialized, costly equipment for analysis of immune cells, eliminating duplication of equipment and effort, making bulk purchases of supplies, and providing skilled laboratory staff experienced in the proposed techniques and protocols. A laboratory Core will also provide consistency of results since all experiments will be performed by the same highly trained group of researchers and staff.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
1P01AI089473-01A1
Application #
8392352
Study Section
Special Emphasis Panel (ZAI1-JRR-I (M2))
Project Start
2012-07-06
Project End
2017-06-30
Budget Start
2012-07-06
Budget End
2013-06-30
Support Year
1
Fiscal Year
2012
Total Cost
$93,623
Indirect Cost
$39,114
Name
Henry Ford Health System
Department
Type
DUNS #
073134603
City
Detroit
State
MI
Country
United States
Zip Code
48202
Wegienka, G; Havstad, S; Kim, H et al. (2017) Subgroup differences in the associations between dog exposure during the first year of life and early life allergic outcomes. Clin Exp Allergy 47:97-105
Cassidy-Bushrow, Andrea E; Sitarik, Alexandra R; Havstad, Suzanne et al. (2017) Burden of higher lead exposure in African-Americans starts in utero and persists into childhood. Environ Int 108:221-227
Sitarik, Alexandra R; Bobbitt, Kevin R; Havstad, Suzanne L et al. (2017) Breast Milk Transforming Growth Factor ? Is Associated With Neonatal Gut Microbial Composition. J Pediatr Gastroenterol Nutr 65:e60-e67
Havstad, Suzanne; Sitarik, Alexandra R; Johnson, Christine Cole et al. (2017) Allergic sensitization in American children of Middle Eastern ethnicity at age 2. Ann Allergy Asthma Immunol 119:464-466
Fonseca, W; Lucey, K; Jang, S et al. (2017) Lactobacillus johnsonii supplementation attenuates respiratory viral infection via metabolic reprogramming and immune cell modulation. Mucosal Immunol 10:1569-1580
Johnson, Christine C; Ownby, Dennis R (2017) The infant gut bacterial microbiota and risk of pediatric asthma and allergic diseases. Transl Res 179:60-70
Sitarik, Alexandra R; Bobbitt, Kevin R; Havstad, Suzanne L et al. (2017) Breast Milk TGF? is Associated with Neonatal Gut Microbial Composition. J Pediatr Gastroenterol Nutr :
Levin, Albert M; Sitarik, Alexandra R; Havstad, Suzanne L et al. (2016) Joint effects of pregnancy, sociocultural, and environmental factors on early life gut microbiome structure and diversity. Sci Rep 6:31775
Cassidy-Bushrow, Andrea E; Havstad, Suzanne; Basu, Niladri et al. (2016) Detectable Blood Lead Level and Body Size in Early Childhood. Biol Trace Elem Res 171:41-7
Lynch, Susan V; Boushey, Homer A (2016) The microbiome and development of allergic disease. Curr Opin Allergy Clin Immunol 16:165-71

Showing the most recent 10 out of 43 publications