The goal of the clinical sample management core is to establish and maintain a collection of highly characterized donors/clinical material (e.g. PBMCs, plasma, bone marrow, saliva, etc) that have been collected, harvested and cryopreserved to support the projects proposed in this application. All cell material is cryopreserved using controlled rate cells freezing techniques that assure viable recovery of subsets at 85% or greater of the number of cells originally cryopreserved. Typically, these clinical samples will be acquired as site specific nested studies that are additions to ongoing clinical trials vaccine or other type of studies (e.g., assessing symptomatic or asymptomafic infecfion). The clinical sample management core regulatory and compliance team will assure that all human subject materials and MTA approvals are in place to permit use of the clinical material in a de identified format. The laboratory operates under College of American Pathologists Accreditation (CAP) and is audited annually for GCLP compliance. All laboratory activifies will be performed following GCLP. Cryopreserved materials will be transported from the Duke clinical sample management core to co-investigator laboratones using LN2 dry shippers.
Influenza is a major public health issue where each year the US experiences at least 30,000 deaths from seasonal presentation of the virus. Pandemic or novel type infections such as the H1N1 virus that appeared in the spring of 2008 can spread rapidly throughout the world with much greater mortality. The work in this clinical core will identify, harvest, and cryopreserve valiable clinical material to be used to support development of a broad spectrum influenza vaccine.
|Kuraoka, Masayuki; Schmidt, Aaron G; Nojima, Takuya et al. (2016) Complex Antigens Drive Permissive Clonal Selection in Germinal Centers. Immunity 44:542-52|
|Xu, Huafeng; Schmidt, Aaron G; O'Donnell, Timothy et al. (2015) Key mutations stabilize antigen-binding conformation during affinity maturation of a broadly neutralizing influenza antibody lineage. Proteins 83:771-80|
|Schmidt, Aaron G; Do, Khoi T; McCarthy, Kevin R et al. (2015) Immunogenic Stimulus for Germline Precursors of Antibodies that Engage the Influenza Hemagglutinin Receptor-Binding Site. Cell Rep 13:2842-50|
|Harrison, Stephen C (2015) Viral membrane fusion. Virology 479-480:498-507|
|Schmidt, Aaron G; Therkelsen, Matthew D; Stewart, Shaun et al. (2015) Viral receptor-binding site antibodies with diverse germline origins. Cell 161:1026-34|
|Jackson, Katherine J L; Liu, Yi; Roskin, Krishna M et al. (2014) Human responses to influenza vaccination show seroconversion signatures and convergent antibody rearrangements. Cell Host Microbe 16:105-14|
|Schmidt, Aaron G; Xu, Huafeng; Khan, Amir R et al. (2013) Preconfiguration of the antigen-binding site during affinity maturation of a broadly neutralizing influenza virus antibody. Proc Natl Acad Sci U S A 110:264-9|
|Dormitzer, Philip R; Grandi, Guido; Rappuoli, Rino (2012) Structural vaccinology starts to deliver. Nat Rev Microbiol 10:807-13|
|Whittle, James R R; Zhang, Ruijun; Khurana, Surender et al. (2011) Broadly neutralizing human antibody that recognizes the receptor-binding pocket of influenza virus hemagglutinin. Proc Natl Acad Sci U S A 108:14216-21|