Work will continue on the isolation and identification of new metabolites of vitamin D and vitamin A found in plasma, tissues and bile of rats and chicks given appropriately labeled compounds. The mechanism whereby metabolites of vitamin D and A are biosynthesized will be studied as will the regulation of these processes using cell culture and enzymological techniques. The mechanism whereby 1,25-(OH)2D3 functions in the target tissues will continue to occupy major interest, especially the isolation to homogeneity of the 1,25-(OH)2D3 receptor protein. Work will be initiated on the differentiation of teratocarcinoma, F9 cells in response to retinoic acid. The gene products produced by the response of these cells to retinoic acid will be studied using two-dimensional gel electrophoresis. The attempted purification of the vitamin K-dependent carboxylase and the study of the vitamin K epoxy reductase will continue. Intermediates in protein processing involved in prothrombin biosynthesis in response to vitamin K will be identified. The pathway of selenium incorporation into glutathione peroxidase will be elucidated. The pro-oxidant effect of Adriamycin and its counteraction or potentiation by vitamin E and selenium will be determined. Work will continue on the relationship between selenium vitamin E and vitamin D intoxication. The possible involvement of dietary manganese in the development of brain damage induced by vitamin E deficiency in chicks work will investigated.

Project Start
1976-05-01
Project End
1990-04-30
Budget Start
1985-05-01
Budget End
1986-04-30
Support Year
15
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
Earth Sciences/Resources
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Demay, M B; Kiernan, M S; DeLuca, H F et al. (1992) Sequences in the human parathyroid hormone gene that bind the 1,25-dihydroxyvitamin D3 receptor and mediate transcriptional repression in response to 1,25-dihydroxyvitamin D3. Proc Natl Acad Sci U S A 89:8097-101
Finlay, J A; DeLuca, H F (1988) Purification and properties of an 18-kilodalton, 1,25-dihydroxyvitamin D3 modulated protein from embryonic chick intestine. Biochemistry 27:3381-7
Uotila, L (1988) Vitamin K-dependent carboxylase from calf liver: studies on the steady-state kinetic mechanism. Arch Biochem Biophys 264:135-43
Cheung, A; Suttie, J W (1988) Synthesis of menaquinone-2 derivatives as substrates for the liver microsomal vitamin K-dependent carboxylase. Biofactors 1:61-5
Suttie, J W; Mummah-Schendel, L L; Shah, D V et al. (1988) Vitamin K deficiency from dietary vitamin K restriction in humans. Am J Clin Nutr 47:475-80
Greer, F R; Mummah-Schendel, L L; Marshall, S et al. (1988) Vitamin K1 (phylloquinone) and vitamin K2 (menaquinone) status in newborns during the first week of life. Pediatrics 81:137-40
Wood, G M; Suttie, J W (1988) Vitamin K-dependent carboxylase. Stoichiometry of vitamin K epoxide formation, gamma-carboxyglutamyl formation, and gamma-glutamyl-3H cleavage. J Biol Chem 263:3234-9
Liska, D J; Suttie, J W (1988) Location of gamma-carboxyglutamyl residues in partially carboxylated prothrombin preparations. Biochemistry 27:8636-41
Ostrem, V K; Tanaka, Y; Prahl, J et al. (1987) 24- and 26-homo-1,25-dihydroxyvitamin D3: preferential activity in inducing differentiation of human leukemia cells HL-60 in vitro. Proc Natl Acad Sci U S A 84:2610-4
Suttie, J W; Hoskins, J A; Engelke, J et al. (1987) Vitamin K-dependent carboxylase: possible role of the substrate ""propeptide"" as an intracellular recognition site. Proc Natl Acad Sci U S A 84:634-7

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