The Keratinocyte Culture Core Lab (KCCL) will provide collaboration, service, and training to the investigators participating in the projects of Interdisciplinary Basic Research in Dermatology. The long-term objective of the KCCL is to serve as a resource of information, skills, and service in in vitro keratinocyte biology for the members of the Program Project Grant.
Specific Aims : 1. Culture normal and pathologic keratinocytes from a number of sources, including newborn foreskin, immortalized cell lines, and skin biopsies. 2. Perform experiments with cultured cells. 3. Maintain special media and provide special techniques for in vitro studies of epidermal metabolism and differentiation. 4. Provide access to and instruction in the use of well-maintained tissue culture facilities and supplies. 5. Train investigators and fellows in the laboratories of principal investigators who wish to learn keratinocyte culture techniques. 6. Develop and adapt new tissue culture techniques to in vitro keratinocyte biology. 7. Maintain the pathologic keratinocyte cell bank. The KCCL is a shared resource staffed by an investigator and technician with special expertise in in vitro keratinocyte biology, responsible to the investigators of Interdisciplinary Basic Research in Dermatology. The skills and research interests of the KCCL members result in strong, collaborative interaction with the investigators of the Program Project Grant. This results in a """"""""product"""""""" that meets the needs of the individual projects and of the Program in a much more complete manner than that offered by a simple service lab.
| Bunick, Christopher G; Presland, Richard B; Lawrence, Owen T et al. (2015) Crystal Structure of Human Profilaggrin S100 Domain and Identification of Target Proteins Annexin II, Stratifin, and HSP27. J Invest Dermatol 135:1801-1809 |
| Chan, Aegean; Godoy-Gijon, Elena; Nuno-Gonzalez, Almudena et al. (2015) Cellular basis of secondary infections and impaired desquamation in certain inherited ichthyoses. JAMA Dermatol 151:285-92 |
| Mitchell, Anna L; Judis, LuAnn M; Schwarze, Ulrike et al. (2012) Characterization of tissue-specific and developmentally regulated alternative splicing of exon 64 in the COL5A1 gene. Connect Tissue Res 53:267-76 |
| Chatterjea, Sudeshna M; Resing, Katheryn A; Old, William et al. (2011) Optimization of filaggrin expression and processing in cultured rat keratinocytes. J Dermatol Sci 61:51-9 |
| Mitchell, Anna L; Schwarze, Ulrike; Jennings, Jessica F et al. (2009) Molecular mechanisms of classical Ehlers-Danlos syndrome (EDS). Hum Mutat 30:995-1002 |
| Abrass, C K; Berfield, A K; Ryan, M C et al. (2006) Abnormal development of glomerular endothelial and mesangial cells in mice with targeted disruption of the lama3 gene. Kidney Int 70:1062-71 |
| Kelsell, David P; Norgett, Elizabeth E; Unsworth, Harriet et al. (2005) Mutations in ABCA12 underlie the severe congenital skin disease harlequin ichthyosis. Am J Hum Genet 76:794-803 |
| Pirrone, Annalisa; Hager, Barbara; Fleckman, Philip (2005) Primary mouse keratinocyte culture. Methods Mol Biol 289:3-14 |
| Presland, Richard B; Fleckman, Philip (2005) Tetracycline-regulated gene expression in epidermal keratinocytes. Methods Mol Biol 289:273-86 |
| Frank, Diane E; Carter, William G (2004) Laminin 5 deposition regulates keratinocyte polarization and persistent migration. J Cell Sci 117:1351-63 |
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