The biochemistry core laboratory will provide technical assistance, services, and advice throughout the duration of the program project for those sub-projects that involve biochemical techniques such as protein/peptide purification, spectrophotometric determinations of proteins/carbohydrates and uronic acid, and gel electrophoresis and densitometry. These techniques are well-established in this laboratory and, as a Core facility, can be used to achieve rapid realization of the goals of these sub-projects. The core will be under the direction of F. Mehraban, Ph.D., who has extensive experience with the various biochemical and immunological technologies and is also the director of a hybridoma Core facility in this department. For those subprojects involving production of antibodies (monoclonal or polyclonal), these can readily be initiated through the established hybridoma core. There will be a full-time experienced research assistant to carry out the diverse techniques involved in the operation of the biochemistry core.

Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106
Hanada, K; Solchaga, L A; Caplan, A I et al. (2001) BMP-2 induction and TGF-beta 1 modulation of rat periosteal cell chondrogenesis. J Cell Biochem 81:284-94
Mansour, J M; Wentorf, F A; Degoede, K M (1998) In vivo kinematics of the rabbit knee in unstable models of osteoarthrosis. Ann Biomed Eng 26:353-60
Hering, T M; Kollar, J; Huynh, T D et al. (1995) Bovine chondrocyte link protein cDNA sequence: interspecies conservation of primary structure and mRNA untranslated regions. Comp Biochem Physiol B Biochem Mol Biol 112:197-203
Hering, T M; Kollar, J; Huynh, T D et al. (1994) Modulation of extracellular matrix gene expression in bovine high-density chondrocyte cultures by ascorbic acid and enzymatic resuspension. Arch Biochem Biophys 314:90-8
Ziran, B H; Pineda, S; Pokharna, H et al. (1994) Biomechanical, radiologic, and histopathologic correlations in the pathogenesis of experimental intervertebral disc disease. Spine (Phila Pa 1976) 19:2159-63