Colorectal cancer is the second most prevalent cancer and the second leading cause of cancer related death in the United States. Botanicals could potentially have anti-cancer compounds that may be used alone or as adjuncts to existing chemotherapy to improve efficacy and reduce the drug-induced toxicity. Identifying herbal sources to develop ideal chemoprevention remains an essential step in advancing the treatment of this cancer and will be addressed in the proposed project. The current proposal postulates based on convincing preliminary results and literature reports that two specific herbs from the ginseng family, steamed or unsteamed roots of American ginseng and notoginseng, may have significant anti-cancer activities.
The aim of this project is to thoroughly characterize the anti-proliferative actions of test herbs and the underlying mechanisms of action in their direct and adjuvant anti-cancer effects. We hypothesize that American ginseng and notoginseng, particularly after steaming preparation, are effective tumoricidal and chemo-adjuvant compounds against human colorectal cancer. In the proposed project, we will test the tumoricidal effects of these two ginseng herbs on distinct human colorectal cancer cell lines and a mouse xenograft model, and determine the involved mechanisms. We will further test whether the extracts potentiate the tumoricidal effects of colon cancer chemotherapeutic agents, 5-fluorouracil and irinotecan, using the cell lines and mouse model. If synergistic interactions between test herbs and chemotherapeutic drugs exist, then it will enable lowering of the drug dose thus reducing the dose-related toxicity. In addition, whether the newly identified active constituents from steamed herbs increase chemotherapeutic agents'activity will be determined. Finally, pharmacokinetic interaction between the chemotherapeutic drugs and the test herbs, if any, will be characterized. A novelty of this project is the use of """"""""knockout"""""""" herbal extracts which will allow a more conclusive association of the effect of the herbal extract to the responsible active constituent(s). Data from the proposed study have a potential to improve treatment regimens and the quality of life for patients suffering from colorectal cancer.

National Institute of Health (NIH)
National Center for Complementary & Alternative Medicine (NCCAM)
Research Program Projects (P01)
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Special Emphasis Panel (ZAT1-SM (11))
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Duffy, Linda C
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University of Chicago
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Wan, Jin-Yi; Wang, Chong-Zhi; Liu, Zhi et al. (2016) Determination of American ginseng saponins and their metabolites in human plasma, urine and feces samples by liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 1015-1016:62-73
Zhang, Qihui; Zhou, Liandi; Chen, Hua et al. (2016) Solid-phase microextraction technology for in vitro and in vivo metabolite analysis. Trends Analyt Chem 80:57-65
Denduluri, Sahitya K; Scott, Bryan; Lamplot, Joseph D et al. (2016) Immortalized Mouse Achilles Tenocytes Demonstrate Long-Term Proliferative Capacity While Retaining Tenogenic Properties. Tissue Eng Part C Methods 22:280-9
Wang, Chong-Zhi; Anderson, Samantha; Yuan, Chun-Su (2016) Phytochemistry and Anticancer Potential of Notoginseng. Am J Chin Med 44:23-34
Lu, Shun; Wang, Jing; Ye, Jixing et al. (2016) Bone morphogenetic protein 9 (BMP9) induces effective bone formation from reversibly immortalized multipotent adipose-derived (iMAD) mesenchymal stem cells. Am J Transl Res 8:3710-3730
Ye, Jixing; Wang, Jing; Zhu, Yunxiao et al. (2016) A thermoresponsive polydiolcitrate-gelatin scaffold and delivery system mediates effective bone formation from BMP9-transduced mesenchymal stem cells. Biomed Mater 11:025021
Wang, Chong-Zhi; Anderson, Samantha; DU, Wei et al. (2016) Red ginseng and cancer treatment. Chin J Nat Med 14:7-16
Wan, Jin-Yi; Wang, Chong-Zhi; Zhang, Qi-Hui et al. (2016) Significant difference in active metabolite levels of ginseng in humans consuming Asian or Western diet: The link with enteric microbiota. Biomed Chromatogr :
Yan, Zhengjian; Yin, Liangjun; Wang, Zhongliang et al. (2016) A Novel Organ Culture Model of Mouse Intervertebral Disc Tissues. Cells Tissues Organs 201:38-50
Mohammed, Maryam K; Shao, Connie; Wang, Jing et al. (2016) Wnt/β-catenin signaling plays an ever-expanding role in stem cell self-renewal, tumorigenesis and cancer chemoresistance. Genes Dis 3:11-40

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